中山大学学报医学科学版

• 基础研究 •    下一篇

β-淀粉样蛋白Aβ25-35诱导HT22细胞自噬及其对V-ATPase表达的影响

张 蓓,谷贝贝,范胜诺,赵 嘉,赵仲艳,刘 军*   

  1. (中山大学孙逸仙纪念医院神经科, 广东 广州 510120)
  • 收稿日期:2012-10-08 出版日期:2013-01-20 发布日期:2013-01-20
  • 通讯作者: 刘 军 E-mail:docliujun@hotmail.com
  • 基金资助:

    国家自然科学基金(30970966);教育部留学归国人员基金(002006006);中央高校基本科研业务费专项基金(11ykpy23)

A β 25-35 Induced Autophagy in HT22 Cells and Relationship with V-ATPase

ZHANG Bei, GU Bei-bei, FAN Sheng-nuo, ZHAO Jia, ZHAO Zhong-yan, LIU Jun*   

  1. (Department of Neurology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China)
  • Received:2012-10-08 Online:2013-01-20 Published:2013-01-20
  • Contact: LIU Jun E-mail:docliujun@hotmail.com

摘要: 【目的】 探讨β-淀粉样蛋白Aβ25-35诱导HT22细胞产生自噬及与V-ATPase 变化的关系?【方法】 将HT22细胞分为对照组?无血清培养组?Aβ处理组和Aβ + Bafilomycin A1组,不同浓度的Aβ25-35作用于HT22细胞24 h,用CCK8测定细胞活力变化,免疫细胞荧光检测自噬斑点变化,及Western blot检测LC3?V-ATPase蛋白变化?【结果】 Aβ可以诱导HT22细胞出现明显的自噬现象,且这种自噬同Aβ的浓度以及作用时间具有一定的相关性?V-ATPase的表达伴随自噬的增多也逐渐增多?Bafilomycin A1可以进一步诱导V-ATPase增加,增加细胞内自噬体的聚积?【结论】 Aβ25-35可诱导HT22细胞产生自噬,且与V-ATPase表达有关?

关键词: Aβ25-35, HT22细胞, 自噬, V-ATPase, Bafilomycin A1

Abstract: 【Objective】To explore Aβ25-35 -induced autophagy in HT22 cells and relationship with V-ATPase. 【Methods】 HT22 cell was divided into control group, serum free group, A β treatment group, and A β + Bafilomycin A1 group. CCK8 assay was used to determine the cell vitality in different concentration of Aβ25-35 for 24 hours. LC3 puncta dots were observed by immune cell fluorescence. The expression of LC3 and V-ATPase were detected by Western blot analysis. 【Results】 Aβ25-35 in HT22 cells effected cell vitality in a time- and concentration-dependent manner. The amount of V-ATPase was correlated with the extent of autophagosome formation. Bafilomycin A1 could further induce V-ATPase and intracellular autophagosome accumulation(P ≤ 0.05). 【Conclusion】Aβ25-35 can induce the autophagy of HT22 cells and is relative with V-ATPase expression.

Key words: Aβ25-35, HT22 cells, autophagy, V-ATPase, Bafilomycin A1

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