中山大学学报自然科学版 ›› 2019, Vol. 58 ›› Issue (5): 114-118.doi: 10.13471/j.cnki.acta.snus.2019.05.014

• 论文 • 上一篇    下一篇

一个新型的药物递送系统——红细胞膜仿生纳米粒

黄际薇,姜彩云,罗宇燕,郭喆霏,张永明   

  1. 中山大学附属第三医院药剂科,广东 广州 510630
  • 收稿日期:2019-02-20 出版日期:2019-09-25 发布日期:2019-09-25
  • 通讯作者: 张永明(1965年生),男;研究方向:药剂学;E-mail: 874477522@qq.com

A novel drug delivery system—red blood cell membranes biomimetic nanoparticles

HUANG Jiwei, JIANG Caiyun, LUO Yuyan, GUO Zhefei, ZHANG Yongming   

  1. Department of Pharmacy,the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China
  • Received:2019-02-20 Online:2019-09-25 Published:2019-09-25

摘要:

结合纳米粒优良的载药特性和细胞膜作为外壳来包载合成的纳米粒内核,使其伪装成内源性物质,减少网状内皮系统的摄取和免疫识别,构建一个新型的药物递送系统——红细胞膜仿生纳米粒递药体系。采用反溶剂法制得纳米粒(nanoparticles, NP),采用离心法提取红细胞膜(red blood cell membranes,RBCM),红细胞膜与纳米粒不同比例共挤压不同次数来制备红细胞膜仿生纳米粒(red blood cell membranes biomimetic nanoparticles,RBCMNP)。通过透射电镜、马尔文粒度仪来表征NP和RBCMNP,利用生物素化纳米粒(biotinylated nanoparticle,BTNP),与链霉亲和素(streptavidin,ST)孵育会发生聚集反应来研究红细胞膜的覆盖程度。NP和RBCMNP粒径均一,优化处方红细胞膜能够完全包裹住纳米粒,重现性良好。

关键词: 纳米粒, 红细胞膜, 仿生纳米粒, 药物递送体系

Abstract:

The study reported a novel drug delivery system, red blood cell membranes biomimetic nanoparticles (RBCM-NP) drug delivery system. The system was constructed by combining the excellent drug-carrying properties of nanoparticles and cell membranes as shells to encapsulate the synthesized nanoparticle which makes it disguise as an endogenous substance to reduce uptake of reticuloendothelial system and immune recognition. Nanoparticles (NP) were prepared by reverse solvent method, while red blood cell membranes (RBCM) were extracted by centrifugal extraction. Different ratios of RBCM and NP were co-extruded for different times to prepare RBCM-NP. RBCM-NP were characterized by transmission electron microscopy and Malvern particle size meter. The aggregation reaction of biotinylated nanoparticle (BTNP) and streptavidin (ST) were used to study the coverage of red blood cell membranes. The particle sizes of NP and RBCM-NP were uniformed. In optimized formulation, RBCM could completely encapsulate NP with good reproducibility.

Key words: nanoparticles, red blood cell membranes, biomimetic nanoparticle, drug delivery system

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