中山大学学报(自然科学版) ›› 2019, Vol. 58 ›› Issue (6): 19-24.doi: 10.13471/j.cnki.acta.snus.2019.06.003

• 论文 • 上一篇    下一篇

不同负荷游泳运动对PM2.5暴露运动大鼠肝脏TNF-α、PPAR-γ蛋白表达的影响

许磊1,汪子铮2,牛亦博3   

  1. 1.山西财经大学体育学院,山西 太原030006;
    2. 山西省体育科学研究所,山西 太原030012;
    3.中国铁路太原局集团有限公司,山西 太原030009
  • 收稿日期:2018-12-19 出版日期:2019-11-25 发布日期:2019-11-25
  • 通讯作者: 牛亦博(1987年生),女;研究方向:运动医学工程技术 ;E-mail:594533031@qq.com

The effect of loading swimming training on the liver protein expression of TNF-α and PPAR-γ for PM2.5 exposured rat

XU Lei1 ,WANG Zizheng2 ,NIU Yibo3   

  1. 1.Institute of Physical Education, Shanxi University of Finance & Economics, Taiyuan 030006,China;
    2.Sports Science Institute of Shanxi, Taiyuan 030012,China;
    3.China Railway Taiyuan Bureau Limited Company, Taiyuan 030009,China
  • Received:2018-12-19 Online:2019-11-25 Published:2019-11-25

摘要:

通过不同负荷游泳训练模式,探讨细颗粒物(PM2.5)暴露下不同运动负荷的大鼠肝脏蛋白表达功能,为PM2.5暴露环境下有氧运动对肝脏功能的影响提供理论依据。建立PM2.5暴露大鼠运动模型,采用随机数字表法分为PM2.5暴露组(暴露组)、PM2.5暴露低负荷组(LES组)、PM2.5暴露中负荷组(MES组)、PM2.5暴露高负荷组(HES组)。采用Elisa法检测脂肪组织肿瘤坏死因子(TNF-α)表达水平;Westernblot检测过化物酶体增殖物激活受体(PPAR-γ)蛋白的表达。TNF-α检测结果显示,与其他组相比,暴露中负荷组TNF-α水平显著性降低(P<0.05);PPAR-γ检测结果显示,与暴露组相比,暴露低负荷组、暴露中负荷组PPAR-γ蛋白表达水平显著性升高(P<0.05);在PM2.5暴露环境下,中等强度游泳训练可有效调节肝脏脂肪功能,抑制炎症状态,从而影响肝脏脂肪功能。

关键词: 游泳训练;不同负荷;sans-serif: ">PM2.5;肝脏;sans-serif: ">TNF-sans-serif: ">α;PPAR-sans-serif: ">γ

Abstract:

This work investigated the protein expression levels of moving rat liver under the exposure of PM2.5  by using different loading swimming training modes. The result provides a theoretical basis for the effects of aerobic exercise on liver function in the exposed environment of PM2.5. The rat training modes under the PM2.5  exposure were set up as follow:  PM2.5  exposure section (E), low exposure section (LES), middle exposure section (MES) and high exposure section (HES) Elisa method was applied to detect the expression level of TNF-α and Western blot assay was used to evaluate the protein expression of PPAR-γ.   The results showed that the TNF-α of MES was lower than that of other sections with the -effective-statistical significance at P<0.05. The  PPAR-γ expression levels of LES and MES were significantly higher than that of E with the effective statistical significance at P<0.05. Therefore, under the exposure of PM2.5 , moderate-intensity training could effectively stimulate the cell factor and promote the signal deliver between Islet cell, as a result, the functions of the liver can be influenced.

Key words: swimming training; different loading;PM2.5; liver;TNF-&alpha, ;PPAR-&gamma

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