中山大学学报自然科学版 ›› 2008, Vol. 47 ›› Issue (3): 140-142.

• 研究简报 • 上一篇    

TaqMan MGB实时荧光PCR对转基因大豆定量检测的研究

黄东东;翁少萍;吕玲;常迪;何建国   

  1. (中山大学生命科学学院,广东 广州 510275)
  • 收稿日期:2007-10-19 修回日期:1900-01-01 出版日期:2008-05-25 发布日期:2008-05-25
  • 通讯作者: 何建国

Detection of Genetically Modified Soybean by TaqMan MGB Realtime PCR

Huang Dongdong;WENG Shaoping; LV Ling;CHANG Di;HE Jianguo   

  1. (School of Life Sciences,Sun Yatsen University,Guangzhou 510275, China)
  • Received:2007-10-19 Revised:1900-01-01 Online:2008-05-25 Published:2008-05-25
  • Contact: HE Jianguo

摘要: 利用实时荧光定量PCR技术,根据转基因大豆(Roundup ReadyTM)的外源基因35S启动子序列设计引物和TaqMan MGB探针,对大豆粉中Roundup ReadyTM大豆含量进行了定量检测,根据这个检测体系建立了35S启动子Ct值与样品中转基因成分数量之间的标准曲线和线性回归方程(相关系数r2: 0.9942)。本研究设计的方法还可以应用到多组分的食品、饲料等加工产品,检测转基因成分的含量,并可作为转基因食品常规PCR定性检测方法。

关键词: 转基因大豆, 实时定量PCR, TaqMan MGB探针, 基因, 生物技术, 食品技术

Abstract: According to the PCR primers and TaqMan MGB probe of exogenous 35S promotor, the quantitative detection of genetically modified soybean ( Roundup ReadyTM) was established by realtime PCR technology. According to this detection system, the standard curve of Ct vs genetically modified organism quantity was generated and a linear regression equation was obtained (r2 : 0.9942). The results demonstrated that this method could be used in quantificational detection of multiple organism food.

Key words: genetically modified soybean, realtime quantitative PCR, TaqMan MGB probe, gene, biotechnolgy, food technology

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