中山大学学报自然科学版 ›› 2010, Vol. 49 ›› Issue (2): 100-104.

• 研究论文 • 上一篇    下一篇

红树林内生真菌的分离及代谢产物生物活性的初步研究

邓祖军1,2,曹理想2,周世宁2   

  1. (1.广东药学院基础学院, 广东 广州 510006; 2. 中山大学生命科学学院//有害生物控制与资源利用国家重点实验室, 广东 广州 510275 )
  • 收稿日期:2009-03-17 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25

Isolation and Metabolite Bioactivities of Mangrove Fungal Endophytes

DENG Zujun1,2, CAO Lixiang2, ZHOU Shining2   

  1. (1. School of Basic Courses, Guang Dong Pharmaceutical University, Guangzhou 510006,China; 2. School of Life Sciences//State Key Laboratory of Biocontrol, Sun Yatsen University, Guangzhou 510275, China)
  • Received:2009-03-17 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

摘要: 从8种红树林植物中共分离出85株内生真菌,经鉴定发现其主要类群为无孢类群(Mycelia sterilia),短梗霉属Aureobasidium spp.和盘长孢属Gloeosporium spp.,分别占菌株总数的50.6%、18.0%、14.6%。对这85株内生真菌的代谢产物生物活性研究发现:其菌丝和发酵液提取物对试验细菌表现出较好的抗性:对大肠杆菌有抗性的菌株比例分别为22.4%、21.2%、金黄色葡萄球菌为12.9%、14.1%,枯草芽胞杆菌为20.0%、36.5%;其发酵液提取物对酿酒酵母Saccharomyces cerevisiae、白色念珠菌Candida albicans、新月弯孢霉Curvularia lunata等试验真菌均有较好的抗性,抗性菌株占菌株总数的比例分别为25.9%,23.5%,28.2%,而菌丝提取物均未表现出抗性;有8株(占菌株总数的9.0%)可产生抑制小麦发芽和芽生长的代谢物,具有除草剂活性;通过本实验建立的全细胞定向筛选模型发现:有14个菌株的代谢产物对耐药性大肠杆菌具有拮抗活性,占菌株总数的16.5%。以上结果表明:红树林内生真菌蕴含丰富的生物活性物质,在新型药物先导物和除草剂的开发方面都表现出潜在的应用前景。

关键词: 红树林, 内生真菌, 代谢产物, 生物活性

Abstract: Eightyfive endophytic fungi strains were isolated from eight kinds of mangrove plants. The main taxa are mycelia sterilia, Aureobasidium〖WTBZ〗 spp. and Gloeosporiumspp., accounting for 18.0%,14.6% of the total strains respectively. Their metabolite bioactivities were primarily studied. Results indicated that the extract of hypha and fermentation liquor have good inhibition activity to test bacteria,the active strains proportion to Escherichia coli is 22.4%,21.2% respectively, to Staphylococcus aureus is 12.9%,14.1%, to Bacillus subtilus is 20.0%,36.5%;fermentation liquor also has good inhibition activity to Candida albicans,Saccharomyces cerevisiae,Curvularia lunata,accounting for 25.9%,23.5%,28.2% of the total strains respectively,but their hypha extract has no inhibition activity to test fungi. Metabolites of eight strains (occupying 9.0% of the total strains) can inhibit germination and bud growth of wheat and have herbicidal activity. Metabolites of fourteen strains(occupying 16.5% of the total strains) can inhibit antibioticresistance Ecoli by wholecell targeted screening model. These results showed mangrove fungal endophytes would be a rich resource pool for new drug and herbicide.

Key words: mangrove plants, fungal endophytes, metabolites, bioactivity

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