中山大学学报自然科学版 ›› 2010, Vol. 49 ›› Issue (2): 65-67.

• 研究论文 • 上一篇    下一篇

高效毛细管电泳安培法测定麦冬中薯蓣皂甙元的含量

黄宝美1,姚程炜2, 莫金垣3, 杜世章1,边清泉1,王志国1   

  1. (1绵阳师范学院化学与化学工程学院,四川 绵阳,621000 2中国空气动力研究与发展中心设备设计及测试技术研究所,四川 绵阳,622653 3中山大学化学与化学工程学院,广东 广州 510275)
  • 收稿日期:2009-07-28 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25

Determination of Diosgenin in Radix Ophiopogonis with Amperometric Detection by Capillary Electrophoresis

HUANG Baomei1, YAO Chengwei2,,MO Jinyuan3DU Shizhang1,BIAN Qingquan1,WANG Zhiguo1   

  1. (1Chemistry and Chemical Engineering,Mianyang Normal University, Mianyang 621000,China; 2Facility Design and Instrumentation Institute of CARDC,Mianyang 622653,China;3School of Chemistry and Chemical Engineering,Sun Yatsen University, Guangzhou 510275,China)
  • Received:2009-07-28 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

摘要: 建立了麦冬中薯蓣皂甙元含量的高效毛细管电泳安培检测方法。着重探讨了缓冲溶液种类、浓度、酸碱度,工作电位,有机改性剂及其操作电压、进样时间等对检测的影响。Na2B4O7NaOH(Na2B4O7浓度为30 mmol·L -1)为缓冲液,工作电位为0.4V,φ为7%甲醇为有机添加剂,进样时间为5 s,在15 kV分离高压,pH=9.5的碱性条件下柱端安培法检测了薯蓣皂甙元的含量。该法的线性范围为:100~1 mg·L -1,线性方程为 Y =17.224 + 8.722 c, 相关系数r=0.999 5,检出限为0.1 mg·L-1。

关键词: 毛细管电泳, 薯蓣皂甙元, 安培检测

Abstract: Diosgenin in radix ophiopogonis was determined by capillary electrophoresis with amperometric detection(CEAD).The affected conditions, such as the running buffer pH value (pH), the types of running buffer, the concentration of running buffer, the detection potential, the applied voltage and the sampling time were investigated. The optimized condition was in a running buffer composed of 30 mmol/L Na2B4O7 buffer at a pH 9.5, separation voltage at 15 kV, injection time for 5 s, organic additive for 7% methanol and detection potential at 0.4 V. A linear relation between peak area(Y) and mass concentration of diosgenin (c) was obtained in the range of 1~100mg·L-1 (r=0.999 5) and the determination limit is 0.1 mg·L-1

Key words: capillary electrophoresis, diosgenin, amperometric detection

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