中山大学学报自然科学版 ›› 2010, Vol. 49 ›› Issue (2): 90-94.

• 研究论文 • 上一篇    下一篇

重金属镉处理的水稻幼根cDNA抑制差减杂交文库的构建

张 美1,袁连玉1,2,刘勋成1,郑 枫1,段 俊1   

  1. (1. 中国科学院华南植物园, 广东 广州510650;2. 中国科学院研究生院, 北京 100039 )
  • 收稿日期:2009-03-12 修回日期:1900-01-01 出版日期:2010-03-25 发布日期:2010-03-25

Construction of Suppression Subtractive Hybridization Libraries with Cadmium Treated Rice Seedling Roots

ZHANG Mei 1, YUAN Lianyu 1, 2, LIU Xuncheng 1, ZHENG Feng 1, DUAN Jun 1
  

  1. (1. South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China; 2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China)
  • Received:2009-03-12 Revised:1900-01-01 Online:2010-03-25 Published:2010-03-25

摘要: 重金属污染是影响水稻生长和稻米品质的重要因素,其中又以镉(Cadmium, Cd)污染最为严重,然而目前对其分子机制以及解决措施却了解甚少。本研究以水稻品种日本晴为材料,采用0.5 mmol/L镉处理水稻幼根48 h,通过运用结合分子镜像选择(mirror orientation selection, MOS)技术的抑制差减杂交(suppression subtractive hybridization, SSH)方法,构建cDNA抑制差减杂交文库,并进行microarray筛选,用于分离应答镉胁迫的差异表达基因。揭示水稻应对镉胁迫处理的分子机理,为水稻耐受镉胁迫基因的分离奠定了基础。

关键词: 镉, 抑制差减杂交, 水稻

Abstract: Heavy metal pollution is an important factor in affecting rice growth and quality of rice. Among all kinds of heavy metal pollution, the cadmium pollution is the most serious one. It is not very clear about the mechanisms of rice responding to cadmium and the measures dealing with pollution. In this paper, to investigate the molecular mechanism of rice response to the Cd treatment (05 mM) and to search for rice genes associated with these mechanisms, suppression subtractive hybridization and mirror orientation selection were conducted to construct cDNA libraries with Cd and nonCd treatment rice (Oryza sativa L., Nipponbare) seedling roots, and cDNA microarray for these two materials was conducted. The results of this study will help to clarify the molecular mechanisms of rice responding cadmium stress, and provide a way of isolating genes involved in heavymetal resistance in rice.

Key words: cadmium, suppression subtractive hybridization (SSH), Oryza sativa L.

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