中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (1): 104-109.

• 研究论文 • 上一篇    下一篇

家兔在发育过程中外侧腓肠肌内MyHCs变化与诱发电位的关系

朱道立, 宋旭甦 , 徐大刚   

  1. (南通大学生命科学学院,江苏 南通 226007∥上海交通大学医学院,上海 200025)
  • 收稿日期:2010-01-25 修回日期:1900-01-01 出版日期:2011-01-25 发布日期:2011-01-25

The Relation of Myosin Heavy Chain Isoform Change and Evoked Potential in the Lateral Gastrocnemius of Rabbits at Postnatal Development

ZHU Daoli,SONG Xushu,XU Dagang   

  1. (School of Life Science,Nantong Univesity,Nantong 226007,China∥ School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China)
  • Received:2010-01-25 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

摘要: 为研究在生后不同年龄组家兔外侧腓肠肌各亚体肌纤维MyHCs变化与诱发电位的关系,探讨生后发育期间肌纤维MyHCs组成和作用差异与表型之间的相关联系,采用电生理记录仪结合十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDSPAGE)检测外侧腓肠肌各亚体。结果表明中间亚体在刺激时各年龄组的峰值电压基本相同,而外侧亚体和内侧亚体分别有些变化。同时发现成年家兔的持续时间都较幼年的长,这可能与各亚体的肌纤维型构成比例有很大关系。外侧腓肠肌各亚体的肌球蛋白重链异构体(MyHCs)电泳条带分别显示MyHCsⅡa、Ⅱd(或Ⅱx)、Ⅱb、Ⅰ共4种,对应于骨骼肌纤维表型ⅡA型(FOG型)、ⅡX(FO型)、ⅡB型(FG型)及Ⅰ型(SO型)4类。

关键词: 外侧腓肠肌, 肌亚体, 诱发电位, 肌球蛋白重链异构体, 腺苷三磷酸酶, 家兔

Abstract: In order to investigate the relation of muscle fiber MyHCs change and evoked potential of each subvolume of the lateral gastrocnemius muscle in rabbits at different postnatal ages,Each subvolume of lateral gastrocnemius muscle in rabbits at postnatal ages were examined by applying the clectronic stimulation instrument and sodium dodecyl sulphatepolyacrylamide gel eletrophoresis(SDSPAGE).The results indicated that only the intermediate subvolume's peak voltage was basically uniform,but the lateral subvolume and the medial subvolume were separately some change. At the same time,it could be seen that grown rabbit's duration was longer than infancy in spite of which subvolume. This was greatly related with the component proportion of fiber types of each subvolume. And eletrophoresis bands of myosin heavy chain isoforms(MyHCs)of each subvolume of the lateral gastrocnemius muscle in rabbits showed respectively MyHCsⅡa,MyHCsⅡd(Ⅱx),MyHCsⅡb and MyHCs I. They corresponded to the muscle fiber phenotype typeⅡA(FOG),ⅡX(FO),ⅡB (FG)and I(SO)fiber.So,the relation between difference of function,the composition of muscle fiber MyHCs and phenotype were approached during postnatal development.

Key words: lateral gastrocnemius muscle, muscle subvolume, evoked potential, myosin heavy chain isoform, actomyosin adenosine triphosphatase, rabbit

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