中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (2): 66-69.

• 研究论文 • 上一篇    下一篇

α-蒎烯促进海洋真菌Aspergillus sp.产真菌毒素青霉酸的研究

李厚金1,谢莹璐1,邱小忠2,蓝文健3   

  1. (1 中山大学化学与化学工程学院,广东 广州 510275;2 南方医科大学解剖教研室,广州 510515;3 中山大学药学院,广东 广州 510006)
  • 收稿日期:2010-11-04 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 蓝文健

αPinene Induces Marine Fungus Aspergillus sp.to Produce Mycotoxin Penicillic Acid

LI Houjin1, XIE Yinglu1, QIU Xiaozhong2, LAN Wenjian3   

  1. (1 School of Chemistry and Chemical Engineering, Sun Yatsen University, Guangzhou 510275, China; 〖JP〗2 Department of Anatomy , Southern Medical University, Guangzhou 510515, China; 3 School of Pharmaceutical Sciences, Sun Yatsen University, Guangzhou 510006, China)
  • Received:2010-11-04 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 采自南海软珊瑚Sarcophyton tortuosum的内生真菌Aspergillus sp.在由葡萄糖10 g/L,蛋白胨5 g/L,酵母膏2 g/L,100%海水,pH为7.5的培养基(GPY)中能够产生真菌毒素青霉酸,产量为5.5 mg/L。在GPY培养基中添加200 mg/L的单萜α蒎烯,能改变代谢产物的组成和含量,并显著促进该真菌产生青霉酸,产量可提高至29.15 mg/L。结果表明α蒎烯在该菌的作用下主要得到系列氧化产物,并进一步发生氧化降解。单萜α蒎烯可以作为一个有效的诱导子,引起微生物的氧化应激反应,改变膜上酶系的活性,使代谢活动得到调控,改变代谢产物组成或产量,在微生物发酵工业上有潜在应用。

关键词: 真菌Aspergillus sp, 青霉酸, α-蒎烯, 生物转化, 诱导子

Abstract: Fungus Aspergillus sp., which was isolated from soft coral Sarcophyton tortuosum, could produce mycotoxin penicillic acid with the yield of 55 mg/L in GPY medium containing glucose 10 g/L, peptone 5 g/L, yeast extract 2 g/L, sea water 1 L, at pH 7.5 Interestingly, while the GPY medium supplied with αpinene at a dose of 200 mg/L, the constitute and content of the metabolites of Aspergillus sp altered obviously Furthermore, the yield of penicillic acid in the culture broth increased dramatically from 5.5 mg/L in control group (GPY medium) to 29.15 mg/L in biotransformation group Based on the gas chromatography mass spectrometry analysis, the major biotransformed products of αpinene were characterized as oxygenated monoterpenoids, which were undergone further biodegradation This finding suggested that monoterpene substrate, αpinene could used to be an elicitor It can alter the oxidase activities, trigger and modulate microbial metabolic pathways, and has potential application in fermentation

Key words: Aspergillus sp., penicillic acid, α-pinene, biotransformation, elicitor

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