中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (2): 75-80.

• 研究论文 • 上一篇    下一篇

鸡血藤提取物中缩合鞣质的含量测定及其抗肿瘤活性初步研究

程 悦1,符 影1,王志宇2,杨得坡1,陈建萍2,王冬梅1
  

  1. (1.中山大学药学院,广东 广州 510006;2.香港大学中医药学院,香港)
  • 收稿日期:2010-12-12 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 王冬梅,陈建萍

Determination on the Contents of Condensed Tannins in Spatholobus suberectus Dunn.Extracts and Primary Study on their Anti-tumor Activities

CHENG Yue1,FU Ying1,WANG Zhiyu2,YANG Depo1, CHEN Jianping2,WANG Dongmei1

  

  1. (1. School of Pharmaceutical Sciences, Sun Yatsen University, Guangzhou 510006,China;2. School of Chinese Medicine, The University of Hong Kong,Hong Kong,China)
  • Received:2010-12-12 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 对鸡血藤/φ=60%醇提物及不同极性溶剂萃取物中总黄酮和缩合鞣质含量进行测定,并测定它们对肿瘤细胞增殖的抑制活性。采用紫外分光光度法测定各样品中总黄酮含量;采用香草醛硫酸法测定各样品中缩合鞣质含量,结果显示,鸡血藤/φ=60%醇提物、乙酸乙酯不溶物、乙酸乙酯萃取物、正丁醇萃取物、水层留余物的总黄酮含量分别为(6.49±1.99)%、(7.36±0.13)%、(9.35±0.22)%、(8.41±0.27)%、(4.48±1.53)%;〖JP〗缩合鞣质含量分别为(5109±0.37)%、(52.34±4.73)%、(73.22±2.72)%、(83.11±5.87)%、(29.74±2.12)%。采用MTT法测定各样品对乳腺癌MCF-7和结肠癌HT-29细胞增殖的抑制活性,结果不同样品对HT-29和MCF-7细胞增殖表现出不同程度的抑制活性,其中正丁醇萃取物对肿瘤细胞增殖的抑制活性最强。

关键词: 鸡血藤, 缩合鞣质, 总黄酮, 抗肿瘤细胞增殖

Abstract: The contents of total flavonoids and condensed tannins in the ethanol extract of Spatholobus suberectus Dunn and its different solvents partitioned extracts were determined by ultraviolet spectrophotometry and VanillinH2SO4 method, respectively. The contents of total flavonoids in the φ=60% ethanol extract, ethyl acetate insoluble extract, ethyl acetate partitioned extract, n-butanol partitioned extract, water residue were (6.49±1.99)%,(7.36±0.13)%,(9.35±0.22)%,(8.41±0.27)%,(4.48±1.53)%, respectively, while their contents of condensed tannins were (51.09±0.37)%,(52.34±4.73)%,(73.22±2.72)%,(83.11±5.87)%,(29.74±2.12)%, respectively. The inhibitory activities on the proliferation of HT-29 and MCF-7 tumor cells were determined by MTT. The extract samples all showed inhibitory activities in different extent on the proliferation of HT-29 and MCF-7 tumor cells. The nbutanol partitioned extract had the strongest inhibitory activities on the proliferation of both tumor cells.

Key words: Spatholobus suberectus Dunn, condensed tannins, total flavonoids, anti-proliferation of tumor cell

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