中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (2): 81-86.

• 研究论文 • 上一篇    下一篇

甲型流感病毒PB1-PA蛋白作用位点的发现与分析

何 淼1,林强乾1,赵艳超1,张淑波1,陈维田2   

  1. (1. 中山大学生命科学学院,广东 广州 510275;2. 香港理工大学屋宇设备工程系,香港 九龙)
  • 收稿日期:2010-03-06 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25

Discovery and Analysis of ProteinProtein Interaction Sites of PB1-PANuclease Complex of Influenza A Virus

HE Miao1, LIN Qiangqian1, ZHAO Yanchao1, ZHANG Shubo1, CHAN Daniel Wai Tin 2

  

  1. (1. School of Life Sciences, Sun Yatsen University,Guangzhou 510275,China; 2.Department of Building Services Engineering, The Hong Kong Polytechnic University,Kowloon,China)
  • Received:2010-03-06 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 基于共进化理论,探究了甲型流感病毒PB1蛋白与PA蛋白上具有共同进化可能性的保守九聚片段 (C9MP)。结构信息显示PB1蛋白的第1-15位氨基酸与PA蛋白的第239-716位氨基酸具有相互作用域;对该区域变异分布的分析发现,PA蛋白第670位氨基酸Q所在的C9MP与PB1蛋白的第9位氨基酸F、第12位氨基酸V和第13位氨基酸P所在的C9MP在PB1-MP1相互作用面上具有最低的共进化值。结合DSSP程序的分析表明,由PA蛋白第670位氨基酸Q与PB1蛋白的第9位氨基酸F、第12位氨基酸V与第13位氨基酸P构成的区域可能成为潜在的相互作用位点。

关键词: 共进化理论, 甲型流感病毒, PB1蛋白, PA蛋白, 相互作用位点

Abstract:

Based on co-evolution theory, this research tries to locate the protein interaction sites in conserved 9mer peptides (C9MP) between PB1 protein and PA protein in the nuclease complex of influenza A virus. There exist interaction regions between sites 1-15 amino acids of PB1 protein and sites 239-716 amino acids of PA protein from structure message. It is revealed by variation analysis to this region that, on PB1-MP1 contact surface, there is a minimum co-evolution value between the conserved 9mer peptide where Q670 of PA protein is located and the conserved 9mer peptide where F9, V12 and P13 of PB1 protein are located. Combined with DSSP analysis results, the regions formed by four amino acids, Q670 of PA protein, F9, V12 and P13 of PB1 protein, are potential interaction sites.

Key words: co-evolution theory, influenza A virus, PB1, PA, interaction site

中图分类号: