中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (2): 99-104.

• 研究论文 • 上一篇    下一篇

中国大鲵5个野生种群的AFLP分析

杨丽萍1, 2,蒙子宁1,刘晓春1,张 勇1,黄俊海3,黄 景3,林浩然1, 4   

  1. (1.中山大学水生经济动物研究所∥广东省水生经济动物良种繁育重点实验室,广东 广州 510275;2. 西南林业大学云南省森林灾害预警与控制重点实验室,云南 昆明 650224;3. 珠海金鲵野生动物养殖有限公司,广东 珠海 519015;4. 海南大学海洋学院, 海南 海口 570228)
  • 收稿日期:2010-04-01 修回日期:1900-01-01 出版日期:2011-03-25 发布日期:2011-03-25
  • 通讯作者: 林浩然

AFLP Analysis of Five Natural Populations of Andrias davidianus

YANG Liping1,2,MENG Zining1,LIU Xiaochun1,ZHANG Yong1,
HUANG Junhai3,HUANG Jing3,LIN Haoran1,4   

  1. (1. Institute of Aquatic Economic Animals ∥ Guangdong Provincial KeyLaboratory for Aquatic Economic Animals, Sun Yatsen University, Guangzhou 510275, China;2. Yunnan Provincial Key Laboratory of Forest Disaster Warning and Control,Southwest Forestry University, Kunming, 650224, China;3. Doumen Golden salamander Aquatic Technology Limited Company, Zhuhai 519100, China;4. Ocean College, Hainan University, Haikou 570228, China)
  • Received:2010-04-01 Revised:1900-01-01 Online:2011-03-25 Published:2011-03-25

摘要: 采用AFLP技术对中国大鲵四川、贵州、湖北、陕西及河南五个野生种群的遗传多样性和遗传分化水平进行了评估。9对引物组合在5个野生种群中共扩增出507条带,其中多态性条带为354。多态位点比例(69.82%)、香农信息指数(0.362 9)和基因多样性指数(0.241 4)等参数均较高,显示中国大鲵遗传多样性水平较为丰富。AMOVA分析发现,中国大鲵遗传多样性绝大部分位于种群内(97.94%)。种群间遗传分化较弱(Фst = 0.021,P<0.001)。根据种群间遗传距离构建的UPGMA树基本依照流域进行聚类,结合化石证据,推测中国大鲵由北向南进行扩散。

关键词: 中国大鲵, 遗传多样性, 遗传分化, AFLP, 系统地理学

Abstract: Genetic diversity and differentiation of natural populations of Andrias davidianus in Sichuan, Guizhou, Hubei, Shanxi and Henan was comprehensively determined using AFLP markers. Sixty individuals from five natural populations were used in AFLP analyses. Nine primer pairs in AFLP analyses produced 507 scorable bands, of which 354 were polymorphic. Three genetic diversity parameters: the percentage of polymorphic loci (69.82%), the Shannons information index (0.362 9) and the Gene diversity (0.241 4) all indicated that A. davidianus was with substantial variation. High level of genetic diversity of A. davidianus agreed with its life history traits and geographical distribution. Most of genetic diversity resided within populations (97.94%) in AMOVA analyses. A low genetic differentiation was characterized by Фst (Фst = 0.021, P<0.001). In the UPGMA dendrogram based on the genetic distance, A. davidianus populations were separated into two major clusters. A. davidianus was supposed to disperse southward from north with the salamander fossil proof.

Key words: Andrias davidianus, genetic diversity, genetic differentiation, AFLP, Phylogeography

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