中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (4): 79-83.

• 研究论文 • 上一篇    下一篇

斜纹夜蛾核多角体病毒诱导甜菜夜蛾细胞凋亡的研究

余 倩1,2
  

  1. (1.仲恺农业工程学院,广东 广州 510225;2.中山大学有害生物控制与资源利用国家重点实验室,广东 广州 510275)
  • 收稿日期:2010-06-06 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25

Apoptosis of Se301 Cells Induced by Spodoptera litura Nucleopolyhedrovirus

YU Qian1,2

  

  1. (1. Zhongkai University of Agiculture and Engineering, Guangzhou 510225, China;2. State Key Laboratory of Biocontrol, Sun Yatsen University, Guangzhou 510275, China)
  • Received:2010-06-06 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

摘要: 斜纹夜蛾核多角体病毒(Spodoptera litura multiple nucleopolyhedrovirus, Splt MNPV)不能成功感染亲缘关系相近的甜菜夜蛾Spodoptera exigua,为了探究其中原因,取Splt MNPV感染甜菜夜蛾离体细胞Se301,利用光学显微镜,电子显微镜以及DAPI(4′,6-Diamidine2′phenylindole dihydrochloride)荧光染色等方法对其进行检测。结果显示Se301细胞被Splt MNPV感染后出现早期凋亡特征,但未进行至细胞凋亡晚期形成凋亡小体;病毒感染受到阻碍,始终没有病毒多角体和有感染性的芽生型子代病毒产生;综合以上结果说明早期细胞凋亡仍然是限制病毒完成复制周期的重要因素。

关键词: 斜纹夜蛾多角体病毒, Se301, 早期凋亡

Abstract: Spodoptera litura nucleopolyhedrovirus (SpltMNPV) could not infect Spodoptera exigua (Se). To investigate the reason of abortive infection, it was detected the pathology of Se301 cells infected by Splt MNPV using different methods, such as microscope, electron microscope and DAPI (4′,6-Diamidine2′phenylindole dihydrochloride) staining, et al.The results demonstrated that the Splt MNPVinfected Se301 cells had obvious pathologic changes deduced as early apoptosis character, but these infected Se301 cells did not process to late apoptosis namely forming apoptosis bodies. There was no infectious BV and ODV in the Se301 cells infected by Splt MNPV all the time, suggesting that early apoptosis was still one of the limited factors in viral replication cycle.

Key words: Splt MNPV, Se301, early apoptosis

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