中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (4): 91-95.

• 研究论文 • 上一篇    下一篇

复合抑制剂对酪氨酸酶活性的相互作用研究

王庆华1,黄演婷1,舒锦华2

  

  1. (1. 广东药学院基础学院//广东省生物活性药物研究重点实验室,广东 广州 510006;2. 广州雅纯化妆品制造有限公司, 广东 广州 510425)
  • 收稿日期:2010-11-17 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25

The Interaction Effects of Combination Inhibitor on Tyrosinase

WANG Qinghua1, HUANG Yanting1, SHU Jinhua2    

  1. (1. The School of Basic Courses of Guangdong Pharmaceutical University//Guangdong Key Laboratory of Bioactive Drugs, Guangzhou 510006, China;2. Grandway International Base Clean Cosmetics Manufacturer, Guangzhou 510425, China)
  • Received:2010-11-17 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

摘要: 研究苯甲酸、肉桂酸和曲酸复合使用对酪氨酸酶活性的抑制以及抑制剂间的相互作用。采用酪氨酸酶L-多巴速率氧化法,观测不同比例的复合抑制剂对酪氨酸酶二酚酶的活性影响,以研究药物相互作用的等效线分析法和相互作用指数评价复合抑制剂的相互作用效应。结果显示在等效于酶活性抑制50%的条件下,苯甲酸与曲酸质量比为7.5∶1,相互作用指数为0.42;肉桂酸与曲酸质量比为3.8:1,相互作用指数为0.56;苯甲酸、肉桂酸和曲酸质量比为7.5∶3.8:1,相互作用指数为0.18。说明苯甲酸、肉桂酸和曲酸复合使用对酪氨酸酶二酚酶活性抑制的相互作用主要表现为协同效应,协同程度与复合抑制剂质量浓度和比例有关。

关键词: 酪氨酸酶, 复合抑制剂, 相互作用, 等效线图

Abstract: In order to study the inhibitive effects of combination of Benzoic Acid, Cinnamic Acid and Kojic Acid on tyrosinase of mushroom,the effects of combination inhibitor on diphenolase activity were observed by measuring the oxidation rate of L-dopa. Isobolographic plot and interaction index were employed to evaluate the inhibitors synergistic effects. The results show that in 50% inhibitory rate of enzyme activity the interaction index was 0.42 when combined with Benzoic Acid and Kojic Acid at the ratio 7.5∶1, the index was 0.56 when combined with Cinnamic Acid and Kojic Acid at the ratio 3.8∶1, and the interaction index was 0.18 when combined with Benzoic Acid, Cinnamic Acid and Kojic Acid at the ratio 7.5∶3.8∶1. Inhibitive effects of the combinaion of Benzoic Acid, Cinnamic Acid and Kojic Acid mainly produce synergism, the mixture concentration and mixing ratio have a great influence on the synergistic effects.

Key words: tyrosinase, combination inhibitor, interaction, isobolographic plot

中图分类号: