中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (4): 96-99.

• 研究论文 • 上一篇    下一篇

饲料能量和维生素B6对乌鳢生长和蛋白质代谢酶活性的影响

王桂芹1,李子平1,牛小天1,芦洪梅1,韩宇田1,赵朝阳2   

  1. (1. 吉林农业大学动物科技学院,吉林 长春 130118;2. 中国水产科学研究院淡水渔业研究中心//农业部淡水鱼类遗传育种与养殖生物学重点开放实验室,江苏 无锡 214081)
  • 收稿日期:2010-08-17 修回日期:1900-01-01 出版日期:2011-07-25 发布日期:2011-07-25

Effect of Dietary Energy and Vitamin B6 on Growth and Enzyme of Protein Metabolism in Channa argus

WANG Guiqin1, NIU Xiaotian1, LU Hongmei1, LI Zhiping1,HAN Yutian1, ZHAO Chaoyang2   

  1. (1.〖JP3〗College of Animal Science and Technology,Jilin Agriculture University,Changchun 130118,China;〖JP〗2.Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes,Ministry of Agriculture ,Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China)
  • Received:2010-08-17 Revised:1900-01-01 Online:2011-07-25 Published:2011-07-25

摘要: 选择体质量(10.92±0.42)g 的健康乌鳢Channa argus为试验鱼。试验一设计3 个蛋白质水平和三个能量水平的9种试验饲料;试验二设计3个蛋白质水平和3个维生素B6水平的9种试验饲料,探讨饲料能量和维生素B6分别与蛋白质的交互作用对乌鳢生长和蛋白质代谢酶活性的影响。结果表明:试验1:在相同饲料能量水平下,鱼体增重随能量水平升高而升高,各器官蛋白酶比活力亦显著提高(P<0.05)。在相同饲料蛋白质水平下,鱼体增重先随饲料能量水平从14.5 MJ/kg上升到16 MJ/kg而升高,随能量水平再升高而降低。饲料蛋白质水平和能量水平都极显著地影响肝脏中GPT的活力(P<0.01),饲料能量水平显著影响肝脏中LDH的活力以及肌肉中LDH和GPT的活力(P<0.05)。蛋能比极显著影响肝脏中GPT的活力(P<0.01)。试验2:乌鳢的生长随着饲料蛋白质水平的增加呈先升高后稳定的趋势,胃、肠和肝脏蛋白酶比活力和肝脏和肌肉中GPT的活力都受到显著地影响(P<0.05),肝脏和肌肉中LDH的活力受到极显著地影响(P<0.01)。随着维生素B6的添加,生长显著增加(P<0.05),胃和肝脏蛋白酶比活力极显著增加(P<0.01)。在同一维生素B6水平下,各器官蛋白酶比活力随着饲料蛋白质水平的升高而显著提高(P<0.05),肝脏和肌肉中GPT和肌肉中LDH的活力亦受到极显著地影响 (P<0.01),肝脏LDH的活力亦受到显著地影响 (P<0.05)。在该试验条件下,适宜蛋能比(23.3 g/MJ)能够提高乌鳢对蛋白代谢酶活力,促进蛋白质代谢,进而促进生长;随着饲料蛋白质含量的增加,适量添加维生素B6(18~30 mg/kg)可促进乌鳢对饲料的利用率,增强蛋白代谢酶的活力,促进蛋白质代谢,进而促进生长。

关键词: 乌鳢, 能量, 维生素B6, 生长, 蛋白质代谢

Abstract: Two trails were conducted to investigate the effects of interaction of protein and energy levels and that of protein and vitamin B6 on growth and enzyme of protein metabolism of snakehead Channa argus(initial body weight (10.92±0.42)g). Results of trail 1 indicated that weight gain of the fish increased with the increase of dietary protein level at same dietary energy level, protease specific activity in determined organs also did (P<0.05).Weight gain of the fish increased with the increase of energy from 14.5 MJ/kg to 16.0 MJ/kg, and then declined with the continuous increase of energy level up to 17.5 MJ/kg at same dietary protein level.Dietary protein and energy level significantly influenced glutamatepyruvate transaminase (GPT) activity in liver (P<0.01).Protease specific activity in determined organs was positively relatated with the increasing dietary protein level at the same dietary energy level. Dietary energy level significantly influenced protease specific activity in stomach and liver (P<0.01). Dietary protein level significantly influenced lactatedehydrogenase(LDH) activity in liver and muscle (P<0.01), dietary energy level significantly influenced glutamatepyruvate transaminase (GPT) activity in liver (P<0.01) moreover significantly influenced lactatedehydrogenase (LDH) activity in liver (P<0.05) and LDH , GPT activity in the muscle of snakehead.Dietary energy level significantly influenced lactatedehydrogenase (LDH) activity in liver and LDH and GPT in muscle (P<0.01). Dietary protein to energy ratio had significant effect on GPT activity in liver (P<0.01). Results of trail 2 indicated that dietary protein levels had significantly improved growth performance then remained stable and significantly influenced on protease specific activity in stomach, Vitamin B6 had significantly influenced growth rate (P<0.05), protease specific activity in stomach and liver (P<0.01).Protease specific activity in determined organs had significantly improved with the increasing dietary protein levels in same B6 level(P<0.05),GPT in liver and muscle and LDH activity in muscle did (P<0.01), LDH activity in liver (P<0.05). In the current experient, the optimal protein to energy radio was 23.3 g/MJ and feeds with adequate vitamin B6 supplementation (18~30 mg/kg) in proper protein level(34.5%~42.0%)could improve growth performance and enzyme about protein metabolism for Channa argus.

Key words: Channa argus, energy, vitamin B6, growth, protien metabolism

中图分类号: