中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (5): 104-109.

• 研究论文 • 上一篇    下一篇

水稻胰蛋白酶抑制剂对褐飞虱产卵刺激的响应

解晓军,高媛媛,戚 舒,周 强
  

  1. (中山大学有害生物控制与资源利用国家重点实验室∥昆虫学研究所,广东 广州 510275)
  • 收稿日期:2010-12-10 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 周强

Rice Bowman Birk Proteinase Inhibitor Response to Oviposition Stimulation of Nilaparvata lugens (St.l)

XIE Xiaojun, GAO Yuanyuan, QI Shu, ZHOU Qiang

  

  1. (Institute of Entomology & State Key Laboratory for Biological Control,Sun Yatsen University,Guangzhou 510275,China)
  • Received:2010-12-10 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要: 昆虫产卵过程对寄主植物的直接刺激包括产卵器的机械损伤和卵表面物质带来影响,这些刺激是启动植物诱导抗虫性的重要途径。通过对褐飞虱产卵处理后水稻植株内胰蛋白酶抑制剂(BBPI)合成酶基因的表达量及物质含量的测定,对比褐飞虱取食和机械损伤处理,发现褐飞虱产卵能够诱导水稻BBPI合成酶基因的表达和BBPI物质的生成。处理后6 h,12 h,水稻 BBPI 表达量显著高于取食和机械损伤处理;产卵诱导的BBPI物质含量显著高于正常水稻。褐飞虱产卵诱导水稻启动〖WTBX〗BBPI〖WTBZ〗表达和物质合成表明,与取食危害类似,水稻同样会对褐飞虱产卵刺激产生响应,启动相应的植物防御体系,进而达到阻滞褐飞虱危害的目的。

关键词: 水稻, 褐飞虱, 产卵, 胰蛋白酶抑制剂

Abstract:

Physical damage caused by ovipoistor and chemicals attached on the surface of insects’ eggs are both included in the direct stimulis of insects’ oviposition in the host plants which are the most important factors in inducing a wide range of defesive responses in plants. This article found that compare with feeding and physical treatments, oviposition of Brown Planthopper (BPH, Nilaparvata lugens (St.l)) can induce the expression of BBPI(Bowman Birk Proteinase Inhibitor) synthetase gene and cause the production of BBPI. Both of the expression levels of BBPI with oviposition treatment after 6 h and 12 h are higher than those of feeding and physical treatments. The results discribed above indicate that as to feeding damage, the rice can react to egg deposition of BPH and induce defensive responses, therefore conctrol the further damage of BPH.

Key words: Oryza sativa, Nilaparvata lugens (St.l), oviposition, bowman birk proteinase inhibitor

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