中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (5): 114-119.

• 研究论文 • 上一篇    下一篇

重症监护病房多重耐药铜绿假单胞菌Ⅰ型整合子的检测及鉴定

毛 璞1,莫红缨2,叶 丹1,刘晓青2,黎毅敏2
  

  1. (1.广州医学院第一附属医院医院感染管理科,广东 广州 510120;2.广州呼吸疾病研究所呼吸疾病国家重点实验室,广东 广州 510120)
  • 收稿日期:2010-12-01 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 黎毅敏

Detection and Characterized of Class Ⅰ Integrons in Multidrug Resistant  Pseudomonas aeruginosa in Intensive Care Unit in Guangzhou, China

MAO Pu1, MO Hongying2, YE Dan1, LIU Xiaoqin2, LI Yimin2   

  1. (1. The Infection Control Department, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China; 2. State Key Laboratory of Respiratory Disease, Guangzhou Medical College, Guangzhou 510120, China)
  • Received:2010-12-01 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要: 整合子是介导细胞多重耐药的重要机制之一,鉴定了2008年某医院重症监护病房分离的23株多重耐药铜绿假单胞菌1型整合子,并应用脉冲场电泳分析其同源性。1型整合子的阳性率达成60.9%。3种1型整合子基因盒被鉴定,其中整合子blaOXA10acc6ⅡcmlA8为首次发现报道。基因盒主要编码氨基糖苷类耐药基因,包括 aacA4, aadA2, aadB, aac6Ⅱ。脉冲电泳结果表明,23株多重耐药铜绿假单胞菌分为5个基因型,A 型(n=5)、B型 (n=6)、C型 (n=4)、D型(n=2)和E型(n=2)。研究表明编码1型整合子是多重耐药铜绿单胞菌较为普遍的特征,且1型整合子与多重耐药表型存相关。研究结果同时也表明防止多重耐药铜绿单胞菌交叉感染仍然是ICU的一项挑战性工作。

关键词: 院内感染, 多重耐药, 铜绿假单胞菌, 1型整合子, 脉冲场电泳

Abstract: To characterize class 1 integrons in multi-drug resistant (MDR) P. aeruginosa in an intensive care unit of a teaching hospital. 23 strain of MDR P. aeruginosa recovered from patients in intensive care unit (ICU) were collected during 2008. The gene cassettes and antibiotic susceptibility of the isolates were characterized. All isolates were also subjected to pulsedfield gel electrophoresis (PFGE). Class 1 integrons were detected in 14 isolates (60.9%). Three types of gene cassettes were identified among these strains, including one that has not been reported previously. The vast majority of the cassettes encoded aminoglycoside resistance gene, including aacA4, aadA2, aadB, aac6Ⅱ.Most of isolates were classified into five PFGE patterns, type A (n=6); type B (n=6); type C (n=4); type D (n=2) and type E (n=2).The present study illustrates that integrons appear to be a common feature among MDR P. aeruginosa in Guangzhou of China, and associated with a high prevalence of antibiotic resistance. Our data also suggested that crossinfection of MDR P. aeruginosa was a challenging work in ICU.

Key words: nosocomail infection, multidrug resistant, Pseudomonas aeruginosa, Class 1 integrons, PFGE

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