中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (5): 87-92.

• 研究论文 • 上一篇    下一篇

香蕉乙二醛酶基因 MaGLO 14 的克隆及在非生物胁迫下的功能鉴定

刘菊华1,邓成菊1,3,金志强1,2,谢学立1, 贾彩红1,张建斌1,徐碧玉1   

  1. (1.中国热带农业科学院热带生物技术研究所∥农业部热带生物技术重点开放实验室, 海南 海口 571101;2. 中国热带农业科学院海口试验站∥香蕉研究所, 海南 海口 570102;3. 云南省红河热带农业科学研究所, 云南 红河 661300)
  • 收稿日期:2010-12-06 修回日期:1900-01-01 出版日期:2011-09-25 发布日期:2011-09-25
  • 通讯作者: 徐碧玉

Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO 14)Under Various Abiotic Stresses

LIU Juhua1,DENG Chengju1,3,JIN Zhiqiang1,2,XIE Xueli1, JIA Caihong1,ZHANG Jianbin1,XU Biyu1

  

  1. (1. Key laboratory of Tropical Crop Biotechnology, Ministry of Agriculture∥Institute of Tropical Bioscience〖JP〗and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,Haikou 571101, China;2. Haikou Experimental Station∥Banana Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 570102, China;3. Institute of Yunnan Tropical Agricultural Sciences, Honghe 661300, China)
  • Received:2010-12-06 Revised:1900-01-01 Online:2011-09-25 Published:2011-09-25

摘要: 为研究香蕉乙二醛酶基因的功能,根据香蕉果实采后早期成熟的抑制缩减杂交文库(suppression subtractive hybridization library,SSH)获得的香蕉乙二醛酶基因片段,首次从香蕉中克隆了乙二醛酶基因的cDNA全长,命名为 MaGLO 14 。该cDNA的ORF全长1 074 bp,编码358个氨基酸。BlastX分析表明,该基因cDNA推导的氨基酸序列与云杉(ABK22263)、葡萄(CBI23235)、葡萄柚(CAB09799)、棉花(ACJ11750)和蓖麻(EEF43857)有较高的一致性,分别为82%、83%、82%、80%、82%。组织特异性表达结果显示,该基因在香蕉根、茎、叶、花、果实中均有表达。在NaCl、低温、乙烯利胁迫处理后基因呈现上调表达;在干旱、涝害胁迫处理后基因呈现下调表达。该基因转化烟草显示能够增强转基因烟草离体叶片耐盐性。

关键词: 香蕉, 乙二醛酶, 克隆, 表达分析, 胁迫, 转基因

Abstract: In order to identify the function of glyoxalase gene in banana, a full length cDNA of glyoxalase gene named MaGLO 14 had been isolated based on the partial sequence cloned by SSH previously from postharvest banana fruit. MaGLO 14 contained a 1 074 bp ORF and encoded 358 amino acids. NCBI Blastx results showed that the deduced amino acid of MaGLO 14 shared identities of 82%, 83%, 82%, 80%, 82% with Picea sitchensis(ABK22263),Vitis vinifera (CBI23235),Citrus paradisi (CAB09799) , Gossypium hirsutum (ACJ11750),and 〖WTBX〗Ricinus communis(EEF43857), respectively. Tissuespecific expression results indicated that MaGLO 14 constitutively expressed in banana root, stem, leaf, flower and fruit. The expression levels were upregulated under NaCl, chilling and ethepon treatments. The expression levels were downregulated under drought and waterlogging treatments. And the MaGLO 14 transgenic tobacco leaves showed enhanced salt-tolerance.

Key words: banana, glyoxalase, clone, expression analysis, stress, gene transformation

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