中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (6): 103-106.

• 研究论文 • 上一篇    下一篇

HBV基因型分析及B/C型共感染的血清病毒种群分析

余 南1,崔 进2,程相华2,周国宝2   

  1. (1.广州萝岗区妇幼保健所中心实验室, 广东 广州 510730;2. 广州开发区医院,广东 广州 510730)
  • 收稿日期:2011-04-08 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25

HBV Genotypic Analysis and Population Analysis of HBV Genotype B/C Co-Infection in vivo

YU Nan1, CUI Jin2, CHENG Xianghua2, ZHOU Guobao2
  

  1. (1.Central Laboratory of Women and Childrens Health Care Institue of Luogang District,Guangzhou 510730,China;2.Guangzhou Development District Hospital,Guangzhou 510730,China)
  • Received:2011-04-08 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25

摘要: 为了解HBV大三阳青年妇女的HBV基因型及体内病毒种群特征,从19~35岁青年妇女HBV感染者血清样本中筛选出24例HBV血清学标志物检测结果为大三阳的样本进行HBV基因分型检测,14例为B型,9例为C型,1例为B/C基因型共感染。对14例大S蛋白基因通过特异性扩增、序列克隆和测序确认的分型结果与分型检测一致。发现的1例B/C共感染样本来自阿德福韦酯抗病毒治疗35周患者,大S蛋白基因序列分析显示10条序列中4条为B型,准种分散值为2.8;其6条为C型,准种分散值为0.6。α决定簇分析该患者体内病毒种群包含3种血清型。病毒种群分析结果提示本地区青年妇女HBV感染的基因型主要为B型和C型,B/C基因型共感染患者体内HBV病毒种群呈复杂的多基因型分布,不同基因型内部以准种形式存在。

关键词: 乙型肝炎病毒, 大S蛋白, “a”决定簇, 准种

Abstract: To investigate genotypes of HBV and the viral population in vivo in young women, we collected serum samples from 24 cases HBV carriers of 19~35 years old young women with both HBsAg and HBeAg positive. Twentyfour samples were tested 14 genotype B, 9 genotype C and one B/C genotypes co-infection. Nucleic acid extracts from 14 serum samples were used to amplify HBV large S protein genes through specific PCR. The amplification products were cloned and ten positive clones of each sample were selected for sequencing. The results confirmed that the 14 samples’ genotype were same. The case of B/C genotypes co-infection was a patient accepted adefovir antivirus therapy for 35 weeks. Four of the ten sequences were of genotype B with DA 2.8. The other six sequences were of genotype C with DA 0.6. There were three serotypes among the ten sequences through “α”determinant analysis. Population analysis suggested that the main genotypes of HBV infected young women in this region were B and C. HBV in vivo might distribute in a complex mode constructed with genotypes as well as quasispecies.

Key words: hepatitis B virus, large S protein, “a” determinant, quasispecies

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