• 研究论文 •

### 基于二级酶失活模型的纤维素酶反应动力学

1. （中国科学院广州能源研究所∥中国科学院可再生能源与天然气水合物重点试验室，广东 广州 510640）
• 收稿日期:2011-06-22 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
• 通讯作者: 袁振宏

### Kinetics of Cellulase Based on the Model of Secondorder Enzymatic Deactivation

ZHANG Yu, XU Jingliang, YU Qiang, LIU Yun, YUAN Zhenhong，QI Wei

1. （Key Laboratory of Renewable Energy and Gas Hydrate∥Guangzhou Institute of Energy Conversion,Chinese Academy of Sciences, Guangzhou 510640, China）
• Received:2011-06-22 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25

Abstract:

Enzymatic hydrolysis of cellulose was extremely complex because of the unclear enzymatic hydrolysis, deactivation mechanisms and many factors that affect the heterogeneous system. Therefore, it is difficult to build a mechanistic model to study cellulose hydrolysis by cellulase. Under some assumed conditions such as the secondorder cellulase deactivation and quasi-steady state theory, a semi mechanistic and empirical model describing the relationship between product concentration and time, the initial enzyme concentration was deduced. The mathematic model was a simple mathematic function that contained only two parameters. The experimental result was in accordance with the deduced mathematic model, where the correlation coefficients (R2) were above 0.98. The relationship between initial enzyme concentration and initial hydrolysis rate calculated from the mathematic model showed good agreement with another type of Henri-Michaelis-Menten equation proposed by Bailey, where the R2 was 0.977 3. The maximum hydrolysis rate and half saturation constant was 2.742 4 g/(L·h) and 3.013 0 g/L, respectively. It was shown from the model that the rate constant of cellulase deactivation decreased when initial enzyme concentration increased under the certain substrate concentration, and the relationship between them was linear. The cellulase deactivation speed increased with the increase of enzyme concentration.