中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (6): 82-87.

• 研究论文 • 上一篇    下一篇

纳米TiO2:C薄膜涂层的构建及对大肠杆菌的抗菌性能研究

麦理想1,张 晟2,王春阳1,谢永建1 ,王大为1,张志光1
  

  1. (1.中山大学光华口腔医学院//附属口腔医院//口腔研究所,广东 广州 510055;2.广东省口腔医院正畸科,广东 广州 510280)
  • 收稿日期:2011-02-21 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
  • 通讯作者: 张志光

Synthesis and Antibacterial Effect Against Escherichia coli of TiO2:C Composite Film Deposited on Titanium

MAI Lixiang1, ZHANG Sheng2, WANG Chunyang1, XIE Yongjian1, WANG Dawei1,ZHANG Zhiguang
  

  1. (1. Guanghua College of Stomatology//Hospital of Stomatology//Institute of Stomatology, SUN Yatsen University, Guangzhou 510055, China;2.Department of Orthodontics, Guangdong Provincial Stamotological Hospital,Guangzhou 510280, China)
  • Received:2011-02-21 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25

摘要: 利用溶胶-凝胶法在纯钛片表面沉积纳米TiO2:C薄膜涂层,采用X射线光电子能谱分析纳米TiO2:C薄膜的化学成分。根据qbt2591-2003国家工业标准测试纳米TiO2:C薄膜对大肠杆菌的抗菌性能,同时通过透射电子显微镜观察细菌被杀灭的超微结构变化过程,分析其抗菌机理。结果表明,在TiO2:C薄膜中C进入锐钛矿型TiO2晶格内并取代Ti或O的位置从而形成O-Ti-C键合,进而抑制TiO2相变。试样对大肠杆菌20 min可见光催化抗菌率均达到90%以上,具有良好的抗菌效果。其杀灭细菌的超微结构变化表明其主要作用于细菌的细胞壁,引起胞壁穿孔,细胞胀大,细胞内容物外溢,直至细菌完全解体。

关键词: 溶胶-凝胶法, 钛表面, 二氧化钛, 抗菌效果

Abstract:

Undoped and Cdoped TiO2 films have been prepared by sol-gel process. The chemical components of the TiO2:C composite thin film were characterized using X-ray Photoelectron Spectroscopy(XPS). The antibacterial efficacy of the TiO2:C composite film was tested by film applicator coating method. Antibacterial mechanism of TiO2:C composite film was discussed by transmission electron microscope(TEM). It has been observed that C dopants retard the transformation from anatase to rutile phase. Namely, C doping effect is attributed to the anatase phase stabilization. More than 90% of bacterium were killed within 20 minutes which confirm fine antibacterial effect of the film.When studying the ultrastructure changes as the antibacterial material of TiO2:C thin film against Escherichia coli,the work indicated that the antibacterial particles of TiO2:C thin film may produce active oxides which contact the Escherichia coli cell and damage even penetrate the cells wall and plasmolemma to enter into the core to make cytoplasm agglomerate and thus cause the cells dead.

Key words: sol-gel process, pure titanium surface, titanium dioxide, antibacterial effect

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