亚致死剂量烯啶虫胺对褐飞虱体内脂肪、蛋白质、可溶性糖及游离氨基酸含量的影响

中山大学学报自然科学版 ›› 2011, Vol. 50 ›› Issue (6): 88-93.

亚致死剂量烯啶虫胺对褐飞虱体内脂肪、蛋白质、可溶性糖及游离氨基酸含量的影响

张洁^1,2，秦小娃²，袁凤辉²，刘杰²，黄婕²，张润杰²

（1.云南省农业生物技术重点实验室∥云南省农业科学院生物技术与种质资源研究所,云南昆明 650223;2.中山大学昆虫学研究所∥有害生物控制与资源利用国家重点实验室，广东广州 510275）

收稿日期:2011-03-02 修回日期:1900-01-01 出版日期:2011-11-25 发布日期:2011-11-25
通讯作者: 张润杰

Effects of Sub-Lethal Concentration of Nitenpyram on Soluble Protein, Glyceride,Free Amino Acid and Soluble Sugar of Brown Planthopper,Nilaparvata lugens (Stå1) (Homoptera: Delphacidae)

ZHANG Jie^1,2, QIN Xiaowa², YUAN Fenghui², LIU Jie², HUANG Jie², ZHANG Runjie²

(1.Biotechnology and Germplasm Resource Institute,Yunnan Academy of Agricultural Sciences,Yunnan Province Key Laboratory of Agricultural Biotechnology,Kunming 650223,China;2.State Key Laboratory of Biocontrol and Institute of Entomology,Sun Yatsen University, Guangzhou 510275, China)

Received:2011-03-02 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25

摘要/Abstract

摘要： 测定了烯啶虫胺亚致死剂量LC₁₀与LC₃₀胁迫12 h与24 h下，褐飞虱5龄若虫与成虫体内可溶性蛋白、糖类、甘油酯及游离氨基酸含量的变化。结果表明：LC₃₀与LC₁₀胁迫下的5龄若虫与胁迫12 h后的成虫体内可溶性糖的含量显著高于对照组；12 h胁迫下，LC₃₀与LC₁₀的烯啶虫胺均能提高5龄若虫与成虫体内游离氨基酸的含量，而24 h处理下的成虫体内游离氨基酸的含量显著低于对照；烯啶虫胺胁迫12 h后，褐飞虱成虫与若虫体内甘油酯的含量显著高于对照，而24 h处理下的5龄若虫体内甘油酯的含量显著低于对照；烯啶虫胺亚致死质量浓度胁迫下的褐飞虱5龄若虫与成虫体内蛋白质含量的变化趋势与游离氨基酸含量的变化有着相似之处。

关键词: 褐飞虱, 烯啶虫胺, 亚致死效应, 可溶性蛋白, 甘油酯, 游离氨基酸, 可溶性糖

Abstract: We compared the changes of soluble sugar, glyceride, free amino acid and soluble protein contents in the 5th instar nymphs and adults of Nilaparvata lugens treated with sub-lethal doses of nitenpyram. The results indicated that the reserves of energy sources in nymphs and adults did have different variation trends. Soluble sugar contents in the 5th instar nymphs treated with LC₁₀, LC₃₀ stress in 12 h and 24 h and adults in 12 h stress were significantly higher than those 0f control; Glyceride contents in 5th instar nymphs and adults treated with LC₁₀ stress were higher than those of control, but under 24 h stress, the glyceride content in the 5th instar nymphs was significantly lower than the control level; The variation trends of soluble protein contents in the 5th instar nymphs and adults were similar to that of free amino acid. The results suggested that under the control of sub-lethal doses of nitenpyram, the 5th instar nymphs and adults may have different coping mechanism and have important significance to understand the mechanism of sublethal doses of insecticide inducing resurgence of Nilaparvata lugens.

Key words: Nilaparvata lugens, nitenpyram, sub-lethal effect, soluble protein, glyceride, free amino acid, soluble sugar

中图分类号:

Q968

张洁;秦小娃;袁凤辉;刘杰;黄婕;张润杰. 亚致死剂量烯啶虫胺对褐飞虱体内脂肪、蛋白质、可溶性糖及游离氨基酸含量的影响[J]. 中山大学学报自然科学版, 2011, 50(6): 88-93.

ZHANG Jie;QIN Xiaowa;YUAN Fenghui;LIU Jie;HUANG Jie;ZHANG Runjie. Effects of Sub-Lethal Concentration of Nitenpyram on Soluble Protein, Glyceride,Free Amino Acid and Soluble Sugar of Brown Planthopper,Nilaparvata lugens (Stå1) (Homoptera: Delphacidae)[J]. , 2011, 50(6): 88-93.

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