中山大学学报自然科学版 ›› 2012, Vol. 51 ›› Issue (1): 72-75.

• 研究论文 • 上一篇    下一篇

钌(Ⅱ)配合物合成、表征与DNA 相互作用及其抗氧化性研究

徐 丽,何 娟,闻伴康,罗简胜,潘堪尚   

  1. (广东药学院医药化工学院,广东中山 528458)
  • 收稿日期:2011-07-09 修回日期:1900-01-01 出版日期:2012-01-25 发布日期:2012-01-25

Synthesis, Characterization, DNAbinding and Antioxidant Activity Studies of Ruthenium(Ⅱ) Complex

XU Li, HE Juan,WEN Bankang, LUO Jiansheng, PAN Kanshang   

  1. (School of Chemistry and Chemical Engineering, Guangdong Pharmaceutical University, Zhongshan 528458, China)
  • Received:2011-07-09 Revised:1900-01-01 Online:2012-01-25 Published:2012-01-25

摘要: 设计合成一个新的钌(Ⅱ)多吡啶配合物[Ru(dip)2(DBHIP)](ClO4)2 {dip = 4,7-二苯基-1,10-邻菲咯啉;DBHIP = 2-(3,5-二溴-4-羟基苯)并咪唑[4,4-f]-(1,10-邻菲啰啉)},采用元素分析,质谱和1H NMR对其进行表征。用电子吸收光谱、黏度测试、Jobplot荧光滴定法研究配合物与CT DNA作用,结果表明配合物以经典的插入模式与DNA键合。采用琼脂糖凝胶电泳实验研究配合物诱导pBR322DNA断裂。同时也研究配合物在高浓度情况下使pGL3 DNA缩合。

关键词: 钌(Ⅱ)配合物, DNA缩合, DNA键合

Abstract: A novel ruthenium(Ⅱ) polypyridyl complex [Ru(dip)2(DBHIP)](ClO4)2 (dip = 4, 7- diphenyl-1,10-phenanthroline, DBHIP = 2-(3,5-dibromo-4-hydroxyphenyl)imidazo [4,5-f][1,10]phenanthroline) was synthesized and characterized. The DNAbinding property of the complex was investigated by spectroscopic methods and viscosity measurement. The results indicated that the complex interacted with DNA through intercalative mode. The photocleavage of pBR322 DNA by Ru(Ⅱ) complex was investigated. The complex induced the aggregation of plasmid pGL3 DNA by Gel retardation assay.The antioxidant activity of the ligand and the complex was also performed.

Key words: ruthenium complex, DNA condensation, DNAbinding

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