中山大学学报自然科学版 ›› 2012, Vol. 51 ›› Issue (2): 59-62.

• 研究论文 • 上一篇    下一篇

一株红树内生真菌Nigrospora sp. 中次级代谢产物及其细胞毒活性研究

魏美燕1,李尚德1,袁宁宁2,梁丽萍1,佘志刚3,林永成3   

  1. (1.广东医学院药学院,广东 东莞 523808;2. 广州工程技术职业学院,广东 广州 510075;3.中山大学化学与化学工程学院,广东 广州 510275)
  • 收稿日期:2011-12-05 修回日期:1900-01-01 出版日期:2012-03-25 发布日期:2012-03-25
  • 通讯作者: 李尚德

Research on the Secondary Metabolites and Their Cytotoxic Activity of Mangrove Endophytic Fungus Nigrospora sp. from the South China Sea

WEI Meiyan1, LI Shangde1, YUAN Ningning2, LIANG Liping1, SHE Zhigang3, LIN Yongcheng3   

  1. (1. School of Pharmacy, Guangdong Medical College, Dongguan 523808, China;2. Guangzhou Institute of Technology, Guangzhou 510075, China;3. School of Chemistry and Chemical Engineering, Sun Yat-sen University, Guangzhou 510275, China)
  • Received:2011-12-05 Revised:1900-01-01 Online:2012-03-25 Published:2012-03-25

摘要: 对采自湛江红树林内生真菌Nigrospora sp.的次级代谢产物进行了首次研究。利用硅胶柱层析、制备薄层层析和重结晶等方法,从该菌培养液的乙酸乙酯相中分离得到3个聚酮类次级代谢产物,运用现代波谱技术鉴定其结构分别为Griseofulvin(1),Vermixocin B (2)和Tenellic acid A (1)。化合物1的立体结构还通过X射线单晶衍射进一步得以确定。体外细胞毒活性测试表明,化合物1对人口腔表皮样癌(鳞癌)细胞KB,人口腔鳞癌多药耐药细胞KBV200,和人肺腺癌细胞A549的细胞毒活性IC50分别为8.23, 9.10和14.26 μmol/L。

关键词: 红树林内生真菌, 次级代谢产物, 细胞毒活性, 单晶X-ray

Abstract: Research on the secondary metabolites of the mangrove endophytic fungus Nigrospora sp. from the South China Sea was reported. Three secondary metabolites were isolated from the ethyl acetate extract of the fermentation by silica gel column chromatography, preparative thin layer chromatography and recrystallgraphy. Their structures were identified by comprehensive spectroscopic methods as Griseofulvin (1), Vermixocin B (2),Tenellic acid A(3). The absolute configuration of compound 1 was further confirmed by singlecrystal X-ray analysis. The cytotoxic activity of compound 1in vitro was also evaluated, and compound 1 showed medium activity against KB,KBV200,and A549 cell lines with IC50 of 8.23, 9.10 and 14.26 μmol/L, respectively.

Key words: mangrove endophytic fungus, secondary metabolites, cytotoxic activity, X-ray diffraction

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