中山大学学报自然科学版 ›› 2016, Vol. 55 ›› Issue (6): 148-152.

• 研究论文 • 上一篇    下一篇

穗花杉双黄酮抑制人脐静脉内皮细胞血管形成的实验研究

张金丽 ,曹文娟 ,熊喜峰 ,戴丽冰 ,刘志河   

  1. 广州市红十字会医院∥暨南大学医学院附属广州红十字会医院创伤外科研究所,广东 广州510220
  • 收稿日期:2016-04-08 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 刘志河;E-mail:zliu0731@163.com

Amentoflame inhibits human umbilical vein endothelial cells angiogenesis

ZHANG Jinli, CAO Wenjuan, XIONG Xifei, DAI Libing, LIU Zhihe   

  1. Guangzhou Institute of Traumatic Surgery,Guangzhou Red Cross Hospital,Medical Collage of Jinan University, Guangzhou 510220, China
  • Received:2016-04-08 Online:2016-11-25 Published:2016-11-25

摘要:

为探讨穗花杉双黄酮(Amentoflame, AF)对人脐静脉内皮细胞ECV304血管形成及机制的影响,从而为穗花杉双黄酮治疗肿瘤及其他血管增生性疾病提供理论基础,采用 MTS检测AF对ECV304血管内皮细胞增殖的作用,细胞划痕实验观察AF对ECV304细胞迁移的影响,体外血管形成实验观察AF对ECV304血管形成的影响,Western blot 检测AF对ECV304细胞血管形成相关信号通路及蛋白如磷酸化AKT(p-AKT)、金属基质蛋白酶9(Matrix metalloproteinase-9, MMP-9)、促血管生成素2 ( angiopoietin 2,Ang 2 )表达影响。结果表明AF能够抑制ECV304细胞的增殖作用,并呈梯度依赖性;AF具有抑制ECV304细胞迁移的作用,并且能够抑制ECV304体外血管样结构的形成;Western blot结果显示100 μmol/L 的AF能够降低ECV304细胞p-AKT、MMP-9、Ang-2的蛋白表达。

关键词: 血管生成, 内皮细胞 , 穗花杉双黄酮

Abstract:

The present study was designed to investigate the effect of Amentoflame (AF) on the angiogenesis of human umbilical vein endothelial cells ECV304 and the mechanisms involved. MTS assay was performed to assess the effect of AF on ECV304 cell proliferation. Wound Healing was performed to assess the effect of AF on ECV304 cell migration. Matrigel assay was performed to assess the effect of AF on ECV304 tube formation. Western blotting was used to analyze the expression of cell angiogenesis related proteins such as angiopoietin 2 (Ang-2)、Matrix metalloproteinase-9 (MMP-9) and p-AKT in ECV304 treated with or without AF. The results demonstrated that AF inhibited the proliferation, migration and tube formation of ECV304 concentration-dependently. Western blot showed that the expression of p-AKT, Ang-2 and MMP-9 were significantly down-regulated by 100 μmol/l of AF.

Key words: angiogenesis, endothelial cells, Amentoflame

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