中山大学学报自然科学版 ›› 2016, Vol. 55 ›› Issue (6): 153-160.

• 研究论文 • 上一篇    下一篇

三株具重金属抗性油菜内生真菌对镉铅锌富集特性研究

徐在超1, 史亚楠1, 张仁铎2, 谢华蓉1, 袁保红1, 王卓娅1, 邓祖军1   

  1. 1. 广东药科大学基础学院,广东 广州 510006; 
    2. 中山大学环境科学与工程学院,广东 广州 510275
  • 收稿日期:2016-03-16 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 邓祖军;E-mail:dengzujun66@163.com

Bioaccumulation of Cd, Zn and Pb by three heavy metal-resistant endophytic fungi isolated from rapes

XU Zaichao1, SHI Yanan 1, ZHANG Renduo 2, XIE Huarong 1, YUAN Baohong 1, WANG Zhuoya 1, DENG Zujun 1   

  1. 1. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China; 
    2. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510275, China
  • Received:2016-03-16 Online:2016-11-25 Published:2016-11-25

摘要:

为了筛选到对重金属具良好富集活性的微生物吸附剂,以分离于大宝山矿区重金属富集植物油菜的3株具重金属抗性的内生真菌Fusarium sp. CBRF14,Penicillium sp. CBRF65 和Alternaria sp. CBSF68为研究对象,分析了Cd、Pb和Zn的初始浓度对其活菌体的生物量、富集率及富集量的影响。研究结果发现,随着重金属初始浓度(试验设置的浓度范围内)的升高,Fusarium sp. CBRF14、Penicillium sp. CBRF65和Alternaria sp. CBSF68对Cd和Zn的富集率呈下降趋势,而对Pb的富集率则存在“先升高再下降”的过程;该3株内生真菌的生物量随着Cd、Pb和Zn初始浓度增加呈下降趋势,而对Cd、Pb和Zn的富集量则不断增加,它们对Cd的最大富集量介于20.5 ~ 53.4 mg·g-1,对Pb的最大富集量介于188.9 ~ 356.6 mg·g-1,对Zn的最大富集量介于28.4 ~ 292.8 mg·g-1。研究结果表明这3株油菜内生真菌可在含重金属液体条件下生长并具有良好的重金属富集能力,其在重金属污染废水的生物修复中具有潜在的应用前景,同时也表明重金属耐性植物内生真菌是获得具重金属富集能力微生物的重要资源库。

关键词: 内生真菌, 生物富集, 生长菌体;重金属

Abstract:

The aim of this study was to select the effective microbial agents to remediate heavy metal pollutants by investigating the potential of the growing biomass of the three endophytic fungi through bioaccumulation to remove  metals from contaminated water. In this study, three endophytic fungi Fusarium sp. CBRF14, Penicillium sp. CBRF65, Alternaria sp. CBSF68 tolerant Cd, Pb, and Zn were isolated from rapes growing in DaBao metal contaminated soils. And then we investigated the effects of initial metals (Cd, Pb and Zn) concentrations on the biomass, the metal bioaccumulation capacities and bioaccumulation percentages of the endophytic fungi. The results showed that the bioaccumulation percentages of Cd and Zn by CBRF14, CBRF65 and CBSF68 decreased with the increase of initial concentrations of Cd and Zn in general, however, the bioaccumulation percentage of Pb by CBRF14 and CBSF68 showed a pattern of “increase-decline”. With the increase of the initial Cd, Pb, and Zn concentrations, the biomass of CBRF14, CBRF65 and CBSF68 showed the declined trend, but the bioaccumulation capacities of metals increased. The maximum bioaccumulation capacities of CBRF14, CBRF65 and CBSF68 were 20.5~53.4 mg·g-1 for Cd, 188.9~356.6 mg·g-1 for Pb, and 28.4 ~ 292.8 mg·g-1 for Zn, respectively. The results indicated that the three heavy metal-resistant endophytic fungi from rapes could live and accumulate metals in solutions with heavy metals, which suggested that they had the potential to remove heavy metals from metal-contaminated water. The results also indicated that plants accumulating multiple metals might harbor diverse fungal endophytes suitable for bioaccumulation of metals.

Key words: fungal endophytes, bioaccumulation, growing mycelium, heavy metals

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