中山大学学报自然科学版 ›› 2016, Vol. 55 ›› Issue (6): 161-164.

• 研究论文 • 上一篇    

尖吻蝮蛇凝血酶分离纯化新工艺研究

邓沁,吴忠,彭维,王永刚,苏薇薇   

  1. 中山大学生命科学学院∥广东省中药上市后质量与药效再评价工程技术研究中心,广东 广州510275
  • 收稿日期:2016-03-30 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 苏薇薇;E-mail:lsssww@126.com

New process for separation and purification of Haemocoagulase Acutus

DENG Qin,WU Zhong, PENG Wei, WANG Yonggang, SU Weiwei   

  1. Guangdong Engineering & Technology Research Center for Quality and Efficacy Re-Evaluation of Post-Market TCM ∥ School of Life Sciences, Sun Yat-sen University, Guangzhou 510275,China
  • Received:2016-03-30 Online:2016-11-25 Published:2016-11-25

摘要:

尖吻蝮蛇凝血酶是从尖吻蝮蛇蛇毒中分离纯化得到的一种类凝血酶,属于止血一类新药。建立了尖吻蝮蛇凝血酶分离纯化新工艺,采用切向流过滤技术,用两步离子交换层析分离得到高纯度的尖吻蝮蛇凝血酶,再采用两步凝胶排阻层析去除热原和脱盐。该工艺具有简便高效的特点,适合大规模工业化生产。

关键词: 尖吻蝮蛇凝血酶, 分离, 纯化, 工艺

Abstract:

Haemocoagulase Acutus (Halase), a new hemostatic in development, is a highperformance thrombin-like enzyme (TLE) derived from snake venom of Agkistrodon acutus. Here we reported a new process established for separation and purification of Halase from snake venom. Combined with tangential flow filtration,  ion exchange chromatography was conducted twice to effectively separate Halase, and two gel exclusion columns were used to remove pyrogen and salt. This new method is simple and efficient, and generates a high purity of Halase, making it a promising process in industrial scale application.

Key words: Haemocoagulase Acutus, separation, purification;process

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