中山大学学报自然科学版 ›› 2018, Vol. 57 ›› Issue (1): 21-28.

• 论文 • 上一篇    下一篇

会仙湿地底泥可培养微生物

阮楚晋1,潘丽霏1,刘洁1,刘祎1,肖咪云1,陆祖军1,2,3   

  1. 1.广西师范大学生命科学学院,广西 桂林541004;
    2.珍稀濒危动植物生态与环境保护省部共建教育部重点实验室,广西 桂林541004;
    3.广西高校野生动植物生态学重点实验室,广西 桂林541004
  • 收稿日期:2017-04-28 出版日期:2018-01-25 发布日期:2018-01-25
  • 通讯作者: 陆祖军(1963年生),男;研究方向:微生物学;Email: luzujun2002@ aliyuncom

Cultured bacteria from the sediment in the Huixian wetland

RUAN Chujin1, PAN Lifei1, LIU Jie1, LIU Yi1, XIAO Miyun1, LU Zujun1,2,3   

  1. 1. College of Life Science, Guangxi Normal University,Guilin 541004,China; 
    2. Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University),Ministry of Education, Guilin 541004,China; 
    3. Key Ecological Laboratory of Wild Animal and Plant Species of Guangxi Colleges and Universities,Guilin 541004,China
  • Received:2017-04-28 Online:2018-01-25 Published:2018-01-25

摘要:

利用平板涂布法分离会仙湿地底泥可培养原核微生物,共获得65个分离株,基于细菌16S rRNA基因序列的相似性和系统聚类分析显示,它们分属4个门,12个属,24个种,包括厚壁菌门的芽孢杆菌属Bacillus、类芽孢杆菌属Paenibacillus、Fictibacillus属、Lysinibacillus属和肠球菌属Enterococcus;放线菌门的链霉菌属Streptomyces和红球菌属Rhodococcus;变形菌门的假单胞菌属Pseudomonas、寡养单胞菌属Stenotrophomonas、泛菌属Pantoea、代尔夫特菌属Delftia以及拟杆菌门的金黄杆菌属Chryseobacterium。分离株中芽孢杆菌属的菌株占53.85%,为优势菌群,其在有机物含量高的条件下会大量繁殖,与湿地富营养化特征相吻合;分离的微生物中含10株4种条件致病性菌,占分离株的15.38%,有机砷耐受菌株30株12种,占46.15%,表明该生态环境已被人为活动严重影响。此外,还分离获得对农药五氯硝基苯(PCNB)耐受性在300 mg/L以上的菌株1株,它们的出现与当地农药与化肥的滥用有着直接关系。

关键词: 会仙湿地, 可培养细菌, 多样性, 富营养化, 耐药性

Abstract:

Culturable bacteria in the sediment of Huixian wetland were isolated by dilution plate coating method and analyzed with 16S rRNA gene sequences. The results showed that total 65 isolates, subordinate to 4 phylums, 12 genera, 24 species, were obtained. These strains belonged to five genera (Bacillus, Paenibacillus, Fictibacillus, Lysinibacillus and Enterococcus) in Firmicutes, two genera (Streptomyces and Rhodococcus) in Actinobacteria , four genera (Pseudomonas, Stenotrophomonas, Pantoea and Delftia) in Proteobacteria and one genus (Chryseobacterium) in Bacteroidetes. The predominant microflora is Bacillus sp. strains, occupying 53.85% of the all the isolates, which conforms to the fact that Huixian wetlands are becoming eutrophic. Totally four kinds of conditional pathogenic bacteria are detected, accounting 15.38% of the isolate, indicating that the ecological environment has been negatively affected by the human activity. There is  another one bacteria strain tolerant to PCNB in 300 mg/L or more, and 30 isolates from 12 Genus have arsenic tolerance. This appearance might have direct relationship with local pesticide and fertilizer abuse.

Key words: Huixian wetland, culturable bacteria, diversity, eutrophication, drug resistance

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