中山大学学报自然科学版 ›› 2019, Vol. 58 ›› Issue (6): 14-18.doi: 10.13471/j.cnki.acta.snus.2019.06.002

• 论文 • 上一篇    下一篇

中华稻蝗共生菌 Fusarium sp. ZMT01固相培养代谢产物

陈嘉晴1,朱水容1, 钟毓1 ,李晓琪1,张小琳1,杨玉莹1,何泽康1,佘志刚2,李春远1   

  1. 1.华南农业大学材料与能源学院,广东 广州 510642;
    2. 中山大学化学学院,广东 广州 510275
  • 收稿日期:2018-11-19 出版日期:2019-11-25 发布日期:2019-11-25
  • 通讯作者: 李春远(1978年生)男;研究方向:天然产物化学;E-mail: chunyuan-li@163.com

The metabolites by solid fermentation from the symbiotic fungus Fusarium sp. ZMT01 of Oxya chinensis Thunber

CHEN Jiaqing1, ZHU Shuirong1, ZHONG Yu1, LI Xiaoqi1, ZHANG Xiaolin1, YANG Yuying1, HE Zekang1, SHE Zhigang2, LI Chunyuan1   

  1. 1. College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China; 
    2. School of Chemistry, Sun Yatsen University, Guangzhou 510275, China  
  • Received:2018-11-19 Online:2019-11-25 Published:2019-11-25

摘要:

研究中华稻蝗肠道共生菌Fusarium sp. ZMT0101 固相发酵代谢产物,用现代层析技术从乙醇提取物中分离单体,波谱技术鉴定结构,二倍稀释法测试抗菌活性。分离到 3-苯基-2-环戊烯-1-酮(1),2-羟基-3-苯基-2-环戊 烯-1-酮(2),曲酸(3),曲酸单甲醚(4),(S)-5-羟基-2,6-二甲基--呋喃[3,4-g]苯并吡喃-4,8(6)-二酮(5),7,8-二甲基苯并蝶啶-2,4(1,3)-二酮(6),(9,11)-8,13-二羟基-9,11-十八碳二烯酸(7),豆甾-7,22-二烯-β,5α,6α-三醇(8)共8个化合物。化合物1,2是首次以天然产物形式分离到,化合物2的核磁数据为首次报道,化合物5~7为首次从Fusarium 属分离到。化合物5对小麦赤霉菌、荔枝炭疽菌显示强抗菌活性。 

关键词: ;中华稻蝗;抗真菌活性

Abstract:

The metabolites by solid fermentation of the symbiotic fungus Fusarium sp. ZMT01 from the intestinal tract of Oxya chinensis Thunber were studied. The molecules were isolated from the ethanol extract by the modern chromatography technology. Their structures were elucidated by spectral methods. The antifungal activities were evaluated using the dilution method. Eight compounds were isolated, they are, are,3-phenylcyclopent-2-en-1-one(1),2-hydroxy-3-phenylcyclopent-2-en-1-one(2),kojic acid(3), kojic acid monomethyl ether(4),(S)-5-hydroxy-2,6-dimethyl-4H-furo[3,4-g]benzopyran-4,8 (6H)-dione(5),7,8-dimethylbenzo [g] pteridine-2,4(1H,3H)-dione(6),(9Z,11E)-8,13-di-hydroxyoctadeca-9,11-dienoicacid.(7)andstigma 7,22-dien-3β,5α,6α-triol(8). Among them, compounds 1 and 2 were first isolated as natural products. The 1H NMR and 13C NMR data of compound 2 were also reported firstly. Compounds 5-7 were obtained from Fusarium sp. for the first time. Compound 5 exhibited high inhibitory activities against Fusarium graminearum Schw. and Colletotrichum gloeosporioides Penz..

Key words: insect symbiotic fungus,  ,  , Fusarium sp.;Oxya chinensis Thunber;antifungal activity

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