中山大学学报(自然科学版) ›› 2020, Vol. 59 ›› Issue (1): 125-132.doi: 10.13471/j.cnki.jsysusse.2020.01.015

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贵州壮侗语族7个少数民族21个Y-SNP多态性

 张秀秀1,2,喻艳琴1,2,田薇1,2,张婷1,2,王婵娟1,2,单可人1,2,何燕1,2   

  1. 1.地方病与少数民族性疾病教育部重点实验室/贵州医科大学,贵州 贵阳 550004;
    2.贵州省医学分子生物学重点实验室,贵州 贵阳 550004
  • 收稿日期:2019-03-25 出版日期:2020-01-25 发布日期:2020-02-28
  • 通讯作者: 何燕(1972年生),女;研究方向:少数民族基因多态性;Email:annieheyan@gmc.edu.cn

Single nucleotide polymorphism of 21 Y-chromosomal loci in seven ethnic minorities of Zhuang-Dong Language in the Guizhou Province of China#br#
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ZHANG Xiuxiu1,2, YU Yanqin1,2 TIAN Wei1,2ZHANG Ting1,2WANG Chanjuan1,2 SHAN Keren1,2HE Yan1,21,2 #br#

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  1. 1.Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education/Guizhou Medical University, Guiyang 550004, China;
    2.Key Laboratory of Molecular Biology, Guizhou Medical University, Guiyang 550004, China
  • Received:2019-03-25 Online:2020-01-25 Published:2020-02-28

摘要: 调查贵州壮侗语族7个少数民族(水族、布依族、侗族、仡佬族、壮族、毛南族、仫佬族)人群 Y 染色体上21个单核苷酸多态性(single nucleotide polymorphism, SNP)位点的遗传多态性,并探讨该人群与其他民族以及其他语族之间的遗传关系。采用 SNapShot法对445例贵州壮侗语族7个少数民族男性个体21个Y-SNP位点进行复合扩增检测,用直接计数法计算等位基因频率、单倍型频率与单倍群频率,运用SPSS 24软件进行主成分分析。在贵州水族、布依族、侗族、仡佬族、壮族、毛南族和仫佬族中,21个Y-SNP组成的单倍型多态性值(haplotype diversity, HD)分别为0.597 7、0.945 6、0.885 6、0.886 9、0.768 6、0.358 7和0.707 8;与已报道的其他9个民族人群单倍群分布频率进行主成分分析,壮侗语族与苗瑶语族聚在一起。从父系遗传角度分析,壮侗语族与苗瑶语族亲缘关系较近。


关键词: 壮侗语族, Y染色体, 单核苷酸多态性, 遗传多态性, 贵州

Abstract: The study aimed to determine single nucleotide polymorphism of 21Y-chromosomal loci in seven ethnic minorities, including Shui, Bouyei, Dong, Gelo, Zhuang, Maonan and Mulam, of Zhuang-Dong language in the Guizhou province of China, and explore differences in the polymorphism from other Language ethnics. The SNapShot method was used to detect the complex amplicons of the 21 Y-SNPs loci in 445 males from seven Guizhou Zhuang-Dong language ethnics. Frequencies of alleles, haplotypes, and haploid populations were calculated using a direct counting method. SPSS 24 was used for principal component analyses. The haplotype diversity values were 0.597 7, 0.945 6, 0.885 6, 0.886 9, 0.768 6, 0.358 7 and 0.707 8, respectively, in the seven ethnics. We performed principal component analyses based on the frequencies of haplogroups by comparing our data with those reported for other nine ethnic groups in China. The results showed that Zhuang-Dong Language and Hmong-Mien Language were clustered together. From the perspective of patrilineal genetics, the relationship of ZhuangDong Language to Hmong-Mien Language is relatively closer than those of other ethnics.


Key words: Zhuang-Dong Language, Y-chromosome, SNP, genetic polymorphism, Guizhou Province

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