Acta Scientiarum Naturalium Universitatis Sunyatseni ›› 2020, Vol. 59 ›› Issue (4): 7-18.doi: 10.13471/j.cnki.acta.snus.2019.10.18.2019E026

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Molecular cloning and expression analysis of heat shock protein 90-β cDNA in jade perch (Scortum barcoo)

GAO Songze, SUN Caiyun, WU Jinying, YE Hangyu, LI Wensheng   

  1. Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic
    Animals / Guangdong Provincial Engineering Technology Research Center for Healthy Breeding of
    Important Economic Fish / School of Life Sciences, 
    Sun Yat-sen University, Guangzhou 510275, China

  • Received:2019-10-29 Online:2020-07-25 Published:2020-07-25

Abstract: Heat shock proteins (HSPs) play important roles in fish stress and immune response, and HSP90-β is an important member of the HSPs family. In the present study, to investigate the possible role of HSP90-β in the immune response, the full-length cDNA sequence of HSP90-β was first cloned from jade perch (Scortum barcoo). The size of the full-length cDNA of jade perch HSP90-β is 2 708 bp, of which 5' UTR is 86 bp, 3' UTR is 444 bp, and ORF is 2 178 bp, which encodes 725 amino acids. The molecular weight of jade perch HSP90-β is predicted as 83 200. And this protein owns five characteristic sequences of the HSP90 family. Moreover, it has no signal peptide and transmembrane domain. Phylogenetic analysis showed that the amino acid sequence identity of jade perch HSP90-β is more than 90% with those of other fishes. Under normal circumstances, the HSP90-β mRNA of jade perch is mainly expressed in the liver. After infection with Streptococcus agalactiae, the HSP90-β mRNA expression decreased first and then increased in liver. Differently, it increased first and then decreased in spleen, head kidney and brain. This result suggests that the HSP90-β may be involved in the immune response elicited by S. agalactiae infection in jade perch.

Key words: Scortum barcooHSP90-β, molecular cloning, expression analysis

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