Acta Scientiarum Naturalium Universitatis Sunyatseni

   

The enzymatic properties of esterase from metagenomic sources and the degradation of phthalates

LIU Yanyan1, LIU Xiaolong2, ZHAO Meng1, FAN Xinjiong1,3   

  1. 1. School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China;
    2. School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230032, China;
    3. School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China


  • Received:2020-03-30 Online:2020-07-03 Published:2020-07-03

Abstract:

Phthalate esters (PAEs) are a kind of important global environmental pollutants. It is particularly important to find a broad spectrum of enzymes that degrade PAEs efficiently and have good environmental adaptability. A novel esterase gene est924 was cloned from microorganisms in soil by metagenomic technique, with the highest homology of 62.42% to the reported alpha/beta-hydrolase derived from Synechococcus sp.cc9311. The gene encoding est924 was obtained by PCR amplification, the recombinant plasmid was obtained by ligating the plasmid pET-41a(+) with est924. Then, the recombinant plasmid was transformed into E. coli BL21 (DE3) for heterologous expression, and the recombinant esterase Est924 was obtained. The enzymatic properties of Est924 and its efficiency to degrade PAEs were studied. The results showed that Est924 obtained in this study was a new alkali-resistant esterase and had good degradation activities to dimethyl phthalate (DMP), diethyl phthalate (DEP), dipropyl phthalate (DPrP), dibutyl phthalate (DBP), diamyl phthalate (DPP) and dihexyl phthalate (DnHP). Therefore, Est924 has a great potential for PAEs bioremediation.

Key words:

esterase, phthalate; metagenomics

CLC Number: