Acta Scientiarum Naturalium Universitatis Sunyatseni

   

The biological target network and underlying mechanisms analysis of Xiaochaihu Granule (XCHG)

BI Cong1, ZHENG Ruwen1, DU Haiyong1, ZHANG Junhua1, JIANG Zhiqiang1HE Yixi1, LIAO Yan2, CHEN Xiuli2, PENG Wei2, YAO Hongliang3, LIU Hong1   

  1. 1. Guangzhou Baiyunshan Guanghua Pharmaceutical Co., Ltd, Guangzhou 510285,China;
    2. School of Life Sciences, Sun Yat-sen University, Guangdong Engineering and Technology Research Center for Quality and Efficacy Re-Evaluation of Post-Marketed TCM, Guangzhou 510275,China;
    3. Guangdong Institute of Applied Biological Resources, Guangzhou 510260,China
  • Received:2020-04-08 Online:2020-07-03 Published:2020-07-03

Abstract:

Xiaochaihu Granule (XCHG) is a famous traditional Chinese medicine (TCM) firstly studied and developed by the Guangzhou Baiyunshan Guanghua Pharmaceotical Co., LTD. The study aimed to investigate the biological target network and potential mechanisms of XCHG through network pharmacology techniques. The compounds and targets of XCHG were screened using TCMSP, PubMed, and UniProt databases. The protein-protein interaction network was analyzed by String and Cytoscape. The GO functions and KEGG pathways were enriched. The active component-target-pathway network of XCHG was established. The results showed that 82 active compounds and 242 potential targets were obtained in XCHG. Wherein, Quercetin, Kaempferol, Luteolin, Wogonin, Naringenin, 7-Methoxy-2-methyl isoflavone, Baicalein, Formononetin, Stigmasterol, and β-sitosterol were the main active compounds; VEGFA, JUN, MAPK3, AKT1, MMP9, IL6, ALB, PTGS2, CXCL8, and CASP3 were the key targets; Twenty key pathways were enriched, and 50% of them were closely related to antiviral effects, such as Human cytomegalovirus infection, Influenza A, Epstein-Barr virus infection, etc. Other major pathways include immune- and inflammation-related pathways such as PI3K-Akt pathway, MAPK pathway, TNF pathway, etc., and apoptosis-related pathways such as Apoptosis pathway, etc. This study showed the advantages of XCHG in suppressing virus replication and viral infection, revealed the key targets and pathways in regulating immune and inflammatory response, and cell apoptosis. The results provided helpful references for XCHG’s mechanism explanation and clinical administration.

Key words:

Xiaochaihu Granule (XCHG), biological target network, antiviral effect, inflammatory reaction, immune response, mechanism

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