›› 2014, Vol. 53 ›› Issue (5): 106-111.

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The Comparative Study on Masculinization of Larvae Nile Tilapia(Oreochromis niloticus)by Immersion in Androgen MT(17α-methyltesosterone)or MDHT (17α-methyldihydrotestosterone)

CHEN Xinghan1,2, LI Bo1, YE Wei3, ZHANG Yong2   

  1. 1. Life Science and Technology Department, Yangjiang Vocational and Technical College, Yangjiang 529566, China;
    2. Institute of Aquatic Economic Animals, Sun Yat-sen University, Guangzhou 510275, China;
    3. Panyu Statelevel Tilapia Breeding Center, Guangzhou 511453, China
  • Received:2014-01-11 Online:2014-09-25 Published:2014-09-25

Abstract: In this paper, we concentrated on masculinization of larvae Nile Tilapia(Oreochromis niloticus)by immersion the larvae in androgens MT(17α-methyltestosterone)or MDHT(17α-methyldihydrotestosterone). The three factors were considered: treatment period(1, 7, 13 DPH, Days Post Hatching), hormone dose(200, 600, 1 800, 5 400 μg·L-1), and treatment duration(2, 4, 8 h). All these three factors were taken into account by simple comparison orthogonal experimental design. The main results are as follows: the 7 DPH larvae induced by MT at a dose of 600 μg·L-1 with 4 h treatment duration is the best result of masculinization, male rate is 98.81%, while at a dose of 1 800 μg·L-1 or 5 400 μg·L-1, male rate greatly decreased(P<0.05); 600 μg·L-1 or 1 800 μg·L-1 dose of MDHT present the best results of masculinization, male rate is 98.34% and 98.39% respectively, at a dose of 200 μg·L-1 or 5 400 μg·L-1, male rate are not significantly decreased(P>0.05) When 7 DPH larvae were induced by MT at a dose of 600 μg·L-1, 4 h is the best treatment duration, 8 h presents greatly decreased result(P<0.05); When MDHT was used, three treatment durations(2, 4, 8 h)got consistent results(P>0.05). The conclusion is that MT and MDHT can both achieve masculinization goals for application of production, but MDHT is more stable and show a better application value.

Key words: Oreochromis niloticus, masculinization, immersion, MT, MDHT

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