The Comparative Study on Masculinization of Larvae Nile Tilapia（Oreochromis niloticus）by Immersion in Androgen MT（17α-methyltesosterone）or MDHT （17α-methyldihydrotestosterone）
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In this paper, we concentrated on masculinization of larvae Nile Tilapia（Oreochromis niloticus）by immersion the larvae in androgens MT（17α-methyltestosterone）or MDHT（17α-methyldihydrotestosterone）. The three factors were considered: treatment period（1, 7, 13 DPH, Days Post Hatching）, hormone dose（200, 600, 1 800, 5 400 μg·L-1）, and treatment duration（2, 4, 8 h）. All these three factors were taken into account by simple comparison orthogonal experimental design. The main results are as follows: the 7 DPH larvae induced by MT at a dose of 600 μg·L-1 with 4 h treatment duration is the best result of masculinization, male rate is 98.81%, while at a dose of 1 800 μg·L-1 or 5 400 μg·L-1, male rate greatly decreased（P＜0.05）; 600 μg·L-1 or 1 800 μg·L-1 dose of MDHT present the best results of masculinization, male rate is 98.34% and 98.39% respectively, at a dose of 200 μg·L-1 or 5 400 μg·L-1, male rate are not significantly decreased（P＞0.05） When 7 DPH larvae were induced by MT at a dose of 600 μg·L-1, 4 h is the best treatment duration, 8 h presents greatly decreased result（P＜0.05）; When MDHT was used, three treatment durations（2, 4, 8 h）got consistent results（P＞0.05）. The conclusion is that MT and MDHT can both achieve masculinization goals for application of production, but MDHT is more stable and show a better application value.