The pollution characteristics and determinants of the atmospheric particulates and its determinants in Guiyang were studied through sorting and statistical analysis of the data of PM2.5, PM10 and meteorological series of Guiyang during 2014-2015, together with assessing of their interaction. The result showed that PM2.5 was the main cause of atmospheric particulate matter in Guiyang, which possessed typical winterspring and summerfall seasonal characteristics. While the average of daily concentration of PM2.5 was 28.79 μg·m-3 in summer, which was fluctuated between 13 to 68μg·m-3. It was increased to 60.64 μg·m-3 in winter, which ranged from 15 to 127 μg·m-3. According to the ambient air quality standard (GB3095-2012), the over standard rate of PM2.5 in spring, summer, fall and winter was 4.4%, 0, 5.5% and 5.5%, respectively. The daily distribution of PM2.5 and PM10 demonstrated a “twin peaks”, the first one of which occurred at 10:00 - 13:00, and it came earliest in summer while latest in winter the second peak occurred at 20:00 - 23:00, the first one of which came earliest in spring while latest in summer. This difference was mainly resulted from the change of season solar radiation and human daily routine. The spatial distribution of PM2.5 showed roughly gradual reduction from urban to rural, the average of which was ordered as Shihuanbaozhan > Yejinting > Huaxi district > Xiaohe district > Hongbianmen > Maanshan > Wudang district > Tongmuling in winter, while the order was Hongbianmen > Yejinting > Xiaohe district > Shihuanbaozhan > Huaxi district > Wudang district > Maanshan > Tongmuling while in summer. Meteorological conditions played a dominant role in atmospheric particulate matter pollution, which was followed by human activities and the local geographical environment.