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Table of Content

    25 May 2016, Volume 55 Issue 3
    Non-stationary flood frequency analysis upon seasonal extremes
    TANG Yihan;CHEN Xiaohong;CHEN Xingzhen
    2016, 55(3):  1-5. 
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    Changes of flood series vary among different seasons. This research developed a flood frequency analysis method based on nonstationary seasonal maximum peak flow. Taking upstream of Wujiang River (UWR) as our study area, seasonal extremes were drawn according to three seasons: dry season (DS) from Nov. to Feb., season of frontal rain (SFR) from Mar. to Jun. and season of typhoon rain (STR) from Jul to Oct. The influences of different seasons on the results of flood frequency analysis were also studied. The results show that: ① Statistical characteristics of different seasons vary in UWR. Mean and contribution to AMS(annual maximum series) (2.22%)of DS are both the lowest while that of SFR are the highest. Standard deviation of STR is the highest. ② All the seasonal series (SS) have ascending trends. Change of SFR causes the most influence on design flood, but the influence is descending. Variation of STR expands the deviation between design floods of different return periods, and the influence is increasing. ③ Design flood from non-stationary flood frequency analysis were larger than the one from traditional method. The one based on SS is larger than AMS, and the difference between increases along with the return period, which is more than 1000 m3/s when return period is 100 years.

    The evaluation and planning implications of urban road network
    YANG Jingyun;HAO Xinhua;ZHOU Suhong
    2016, 55(3):  6-14. 
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    In depth study of the use of the road system, so as to carry out an empirical test by using the current situation of traffic operation on the road design specifications and related indicators. Evaluating the urban road network by using the massive objective spatiotemporal data will help to summarize the rationality of the existing transportation planning concept, the related technical basis and the design specifications. Using the floating car data of Shenzhen city, the distribution of urban traffic flow, its affecting factor as well as the relationship with road construction are analyzed from three aspects, including the road network, different levels of roads, and roads intersection. The results show that: ① Factors affecting the spatio-temporal distribution of traffic flow include the time, road itself attributes, location factors, the speed of higher level road connected directly. ② By analyzing the speed of different types of road length ratio, we find that actual operating speed of sub-distributors is low which indicates there is some irrationality in Road Design Standard. ③ The intersections of urban roads usually affect the near traffic flow within 100 meters, and the average queue length of intersections is about 35~40 m.

    Drought disaster evaluation model and characteristics of late rice in Guangdong
    CHEN Huihua;WANG Chunlin;DUAN Hailai;FAN Shaojia
    2016, 55(3):  15-21. 
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    Using yield and growth period data of late rice, drought index of each growth period was calculated by daily meteorological drought indicator (DI), and drought disaster evaluation model was established and temporal-spatial characteristics of the drought disaster was analyzed in this paper. Main results are: ① Average drought frequency of late rice in Guangdong during recent 30 years (1981-2010) was 6.9%; mean yield reduction rate was 0.7%. Drought disaster mainly occurred at milk to mature stage of late rice, and light drought day's accounted for the main. ② Spatial distribution of late rice drought frequency and yield reduction rate was high in the north and low in the east of Guangdong. High incidence region of drought located in northwest of Guangdong, where the drought frequency was 15%~25%, and yield reduction rate was 1%. ③ Drought disaster of late rice in Guangdong tended to decrease without statistical significance during recent 30 years, which tended to increase in the southeast while decrease at the other regions. ④ Drought frequency and reduction rate of late rice tended to decrease under climate change. Therefore, the key period of the late rice drought disaster in Guangdong is heading to milk stage, and the attentions should be paid more on the northwest of Guangdong.

    Transport characteristics of surface sediments at the Yangtze river estuary
    CHEN Dinghao; REN Jie;TANG Quanhui;WEN Li
    2016, 55(3):  22-29. 
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    Cluster analysis and Gao-Colliins particle size trend analysis model will be used in sediment characteristics and transport of Yangtze Delta 948 surface sediment samples. The results showed that: Changjiang Estuary sediments can be divided into three categories. The first class of sediments composed mainly of silt, these are mainly distributed in the Yangtze Delta southeast 10 m depth contour to 40 m isobath range. The second category mainly silty sediment sand, sandy silt, mainly distributed in the waters east of Qidong between 20 to 40 m bathymetric contour. Yangtze River estuary sediment transport in the overall trend can be broadly divided into two directions toward the estuary of northeast and the coast of south. North branch of the Yangtze River near the exit of the sediments have a tendency to transport within the estuary. North branch of estuary sediments isobath 20~40 m between the transport to the northeast. Southern branch outlet affected runoff, coastal streams south transport part, the other part shelf seaward transport and counterclockwise turn to the northeast in the 10 m isobath sediment transport trends section, in the northeast corner of the study area there is transport to the estuary of the trend shelf.

    Spatio-temporal variation of average temperature over the Pearl River basin during 1959-2013
    XIE Yiwen;LI Juan;CHEN Weirong;LUO Shihao
    2016, 55(3):  30-38. 
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    Based on the monthly average temperature data from 43 meteorological stations during 1959 to 2013, the spatiotemporal variation in the Pearl River basin was analyzed using the methods of linear regression, Mann-Kendall and wavelet analysis. The results show that: the average temperature showed an increasing trend in different time scales and the average temperature in most of the areas increased significantly (P<0.05). The annual average temperature series of the Pearl River basin had a mutation in 1993 but the other time scales had mutations mainly in the early 1990s. The primary period of the annual average temperature series was 3.6a and there were 2.4~3.4 years and 2.8~4.4 years change periods in 1963-1970 and 1983-2008 respectively. The value range of the average temperature of the basin was 10.68~22.60℃ with the average value of 19.50℃, which showed an increasing trend totally form the western to eastern. The average temperature of most meteorological stations of different time scales showed an increasing trend, which increased significantly in the Pearl River Delta and the Dongjiang River basin due to the influence of urbanization.

    The spatial and seasonal variations of partial pressure of carbon dioxide in surface water in Pearl River Estuary
    MA Yu;GAO Quanzhou; LI Tuanjie;TANG Wenkui;ZHANG Qianzhu;SHI Huaming
    2016, 55(3):  39-46. 
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    The focal area of this study is the Pearl River Estuary(PRE). In this study, the surface partial pressure of carbon dioxide pCO2, temperature, salinity and dissolved oxygen (DO) were determined by underway measurement system during four field surveys, and their seasonal and spatial variations were shown. Significant seasonal variations of (pCO2)was observed in the upstream region of PRE, pCO2 in summer was the lowest at the same salinity, medium in spring and fall, the highest in winter, which indicated that the weatheringderived dissolved inorganic carbon was diluted in wet season and sea water was more close to upstream region in dry season. High pCO2 and low DO occurred in the upstream region during four seasons, andpCO2 and DO showed a strong negative linear correlation, and aerobic respiration was the most important factor in modulating high pCO2. Especially, the maximum of pCO2 was 717.2 Pa and the minimum of DO was 41.4 μmol/L when salinity was 0.19‰ in spring. Pearl River runoff discharged an amount of nutrients into nearshore waters and promoted primary production in broad region in spring and summer. Phytoplankton photosynthesis produced oxygen into sea water and removed carbon dioxide from sea water. The lowest pCO2, 16.5 Pa and the highest DO, 418.8 μmol/L existed in the western of Hong Kong where salinity ranged from 15‰ to 25‰ in summer.

    Global stability in a SQIR epidemic model with ecological environment and stage structure
    FU Jinbo;CHEN Lansun
    2016, 55(3):  47-51. 
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    By using epidemic dynamic theory, a class of SQIR epidemic model with ecological environment and stage-structure is established, in which the population is divided into two life stages-mature and immature, and the viruses spread only in adult population, while the susceptible group of adult population and active sub adult group of immature population are insulated from the infected area by adopting control strategy. By means of qualitative method and stability method of ordinary differential equations, the boundedness of the model and the existence of nonnegative equilibrium point are analyzed. By constructing proper Lyapunov function and limit system theory, sufficient conditions of the global asymptotic stability of the trivial equilibrium point, disease-free equilibrium point and endemic equilibrium point are obtained. The results show that: when the basic reproduction number is less than or equal to 1, all populations tend to be extinct; when the basic reproduction number is greater than 1 and satisfy certain conditions, the viruses will be cleared; when the dominant regeneration number of the viruses is greater than 1 and satisfy certain conditions, the viruses continue to prevail and will become a local disease.

    Measure center and minimal contracting center for amenable group actions
    WANG Lijuan;ZHOU Yunhua
    2016, 55(3):  52-54. 
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    The measure center and minimal contracting center for amenable group actions are investigated. More precisely, the definitions of the measure center and the minimal contracting center for amenable group actions are given firstly. Then the main result that the measure center of a nonempty set equals to its minimal contracting center is proven. It generalizes the conclusion of Zhou to amenable group actions.

    The uniformly asymptotic estimate for the tail probability of the sums of nonnegative and dependent random variables
    TANG Fengqin
    2016, 55(3):  55-58. 
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    Suppose that {Xi}i≥1 is a sequence of nonnegative and nonidentically distributed random variables which belong to the subclass of heavy-tailed distributionsclass C. The multivariate distribution function of the random variables is governed by the FGM copula function. The uniformly asymptotic estimate for the partial sums and random sums of the sequence {Xi}i≥1 are studied, respectively. The obtained results extend the corresponding asymptotic estimate of the tail probability of the dependent random variables.

    The generalization of high order Haar functions
    CHEN Wei;CAI Zhanchuan
    2016, 55(3):  59-63. 
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    The k degree V-system is a class of orthogonal piecewise polynomial functions which is also named as high order Haar functions. V-system is defined on the uniform partition of interval [0,1]and obtained by multiscale squeezing and shifting operations on the so-called generators. The V-system to the case of non-uniform partition is generalized, and the corresponding result is named as high order non-uniform Haar functions. For any given partition on the interval [0,1], a set of truncated monomials was firstly defined. It is proved that the non-uniform Haar functions can be obtained through the Gram-Schmidt orthogonalization process. The orthogonality, reproducibility and convergence of the proposed functions are proved, and a specific constructive example is also given.

    The existence of steady-state positive solutions for a spider-insect model
    JIANG Hongling
    2016, 55(3):  64-67. 
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    A one-prey-two-predator spider-insect model is studied. By the variational principle of eigenvalue and the Maximum principle, priori estimates and the necessary conditions of existence for positive solutions are given. Applying the method of space decomposition and implicit function theorem, a sufficient condition of existence of positive solutions is obtained. The results show that, under certain conditions, the coexistence depends on the growth rate of insects.

    Dynamic stability research on rectangular thin plate based on the quadratic eigenvalue method
    ZHONG Zilin, LIU Airong,LU Hanwen, HUANG Youqin
    2016, 55(3):  68-76. 
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    Based on the Von-Karman large deflection theory of thin plates, the second-order ordinary differential Mathieu-hill Parametric vibration equation was established for of simply supported rectangular thin plate under in-plane periodic loading using the Galerkin method. The quadratic eigenvalue method was adopted to obtain the main and secondary instability domains of the rectangular thin plate with the periodic solutions of 2T and T, then the accuracy of the method was verified by finite element method, meanwhile, the influence of nonlinear elasticity on stationary amplitude caused by the main Parametric resonance was qualitatively analyzed. The analytic results showed that strong transverse parametric resonance will occur when the frequency of excitation force is about twice as large as the natural vibration frequency of the thin plate. The quadratic eigenvalue method can be used to calculate accurately those parameters, such as the frequency and the excitation coefficient of the dynamic instability region of the rectangular thin plate. As the increasing of the amplitude, the infinite increase of vibration amplitude was controlled owing to the nonlinear factor which tows system to the direction of large vibration frequency. Furthermore, the nonlinear factor can bring the system into a complex vibration state in which the vibration amplitude increased stably or rapidly.

    An image retrieval algorithm based on hierarchical compressive sensing in HSV space
    ZHOU Yan;ZENG Fanzhi;ZHAO Huimin
    2016, 55(3):  77-82. 
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    By constructing a two-dimensional (2D) compressive sensing (CS) measurement model, a new image retrieval algorithm is proposed by extracting hierarchical HSV features and texture features. Firstly, the ideas of grid discrete partition and hierarchical mapping in HSV space are introduced, and hierarchical mapping matrix and similar GLCM in HSV grid space are defined. Secondly, the normalized Gauss random matrix is designed as measurement matrix, and compressive sampling on the above two matrixes is performed by 2D CS measurement model. With using PCA(Principal Component Analysis), the feature sequences as hierarchical HSV features and texture features are obtained from the above two hierarchical sampling matrixes. Finally, the above two features are infused to compute the overall similarity among images. Experimental results show that the above two features have good discrimination. It can improve the efficiency for image retrieval, and has good performance especially for images with complex backgrounds.

    The new solution and numerical emulation for a nonlinear RLC circuits
    HUANG Cai;YU Sunzheng
    2016, 55(3):  83-88. 
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    A new solution and its numerical emulation for the nonlinear RLC circuit are given. The state variable of the circuit is expressed as the simple harmonic function of the phase angle. The determination for the first derivative of the phase angle about the time are used to solve the state equations. The relation of the time independent variable with the middle variable of the phase angle is shown as the integral for the reciprocal of the response frequency. The numerical values, including  the phase portraits, the time-distance curve, the phase-distance curve, and the time-range curve, and the phase-range curve, and the range-frequency curve, and the phase-frequency curve and the response period, are computed. The results are in good agreement with the numerical integral method.

    Soliton excitation in a one-dimentional antiferromagnetic-chain with bond alternation
    WANG Sen,CHEN Hao,WANG Ruiqiang
    2016, 55(3):  89-96. 
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    The soliton excitation of a onedimensional Heisenberg antiferromagnetic chain with bond alternation was studied. In the Hamiltonian of this antiferromagnetic model, the interactions between the nearest neighbor lattice and next nearest neighbor lattice,Dzyaloshinskii-Moriya interaction and constant external magnetic field were considered. By using Holstain-Primakoff transformation and the multiscale method, spin wave frequency ω was obtained when nonlinear term in the system was neglect, and two bright soliton solutions of the two sets of sublattice were obtained when nonlinear term was considered. Finally, the effects of the wave vector k and bond alternation strength m ( the nearest exchange interaction parameter ratios J′1/J1)on ω,the soliton peak value,width and energy were discussed. The results show that ω can be divided into optical branch ω+ and acoustic branch ω. ω does not exist near kb=0,±2π(b is the distance between the two nearest neighbor cells). Acoustic branch ω corresponds to bright soliton solution, optical does not. When J1 is certain, the smaller the m is, the smaller the gap between ω+ and ω will be. The peak width,phase velocity,group velocity and energy quantum of the two bright soliton solutions are of the same values,but the envelop amplitude and phase are different with the same parameter. The energy quantum of the soliton solutions is smaller than the energy quantum of the spin wave solution when nonlinear term is not considered, so the two soliton solution is stable. The m affects not only the relative peak value, but also the relative phase of the two solitons.The peak value of two soliton solutions vary with the changes of m and k, but the change is not the same. The peak width does not increase monotonously with the crease of k, nor does it increase monotonously with that of m.

    Lie symmetry and conserved quantity of fractional Lagrange system based on El-Nabulsi models
    ZHANG Xiaocai;ZHANG Yi
    2016, 55(3):  97-101. 
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    The Lie symmetry and the conserved quantity of fractional Lagrange system based on El-Nabulsi models are studied. Firstly, the D-Alembert-Lagrange principle of the El-Nabulsi models is deduced based on the fractional action-like variational problem which is expanded by the Riemann-Liouville integral, and the differential equations of motion of the system are obtained. Secondly, the definition and the criterion of the Lie symmetry are given, the determination equations of the Lie symmetry of the system are established, and the generalized Hojman theorem is put forward. At the same time, the existence condition and the form of the generalized Hojman conserved quantity are obtained. Then, the generalized Noether theorem is established, the existence condition and the form of the Noether conserved quantity led by the Lie symmetry are given. Finally, two examples are given to illustrate the application of the results.

    Structural damage identification including the temperature difference based on response sensitivity analysis
    ZHANG Chunli; LV Zhongrong
    2016, 55(3):  102-105. 
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    Damage detection based on a reference set of measured data usually has the problem of different environmental temperature in the two sets of measurements, and the effect of temperature difference is usually ignored in the subsequent model updating. It attempts to identify the structural damage including the temperature difference both local damages, and the temperature difference are identified in a gradientbased model updating method based on dynamic response sensitivity. The measured dynamic responses of the structure from two different states are used directly to identify the structural local damages and the temperature difference. A planar truss structure are studied to illustrate the effectiveness of the proposed method.

    Quantitative determination of multiple elements in Rosa roxburghii and Rosa sterilis fruits using ICP-OES with microwave digestion
    ZHANG Yuanhe;MA Na;HE Jingyu;GUAN Yanyan;HUANG Yu;YUAN Huijie;LI Song;LAI Zhihui
    2016, 55(3):  106-110. 
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    We have established a method for determination of the trace elements within Rosa roxburghii and Rosa sterilis Fruits in this study. The contents of 15 elements (Al, B, Ba, Ca, Cu, Fe, K, Mg, Mn, Na, Ni, P, S, Sr, Zn) were determined by ICP-OES with microwave digestion. The linear ranges of the elements were ranged from 0~10 mg/L with a correlation coefficient more than 0.999 1. The detective limits of the instrument were ranged from 0.000 1~0.006 1 mg/L, precision experiments showed RSD ranged from 0.3%~2.7% and the recoveries of the elements were in the range of 93.3%~107.7%. Such method was used to test the national standard substance Astragalus membranaceus (GBW 10028), and the result was consistent with the certificate value. Therefore, this method can be used for quickly and accurately measuring trace elements within Rosa roxburghii and Rosa sterilis Fruits.

    Qualitative and quantitative analysis of dissolved matters of eucalyptus leaves by solid phase microextraction coupled with gas chromatography-mass spectrometry
    HUANG Bensheng;HONG Changhong;XU Mingzhi;LUAN Tiangang;WANG Xiaowei
    2016, 55(3):  111-116. 
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    In order to reveal the effects of dissolved matters of eucalyptus leaves to the aquatic environments. Solid phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GCMS) was selected for qualitative and quantitative determination of dissolved matters of eucalyptus leaves in pure water during different immersing times in this study. By searching NIST database, 139 different chemicals are identified with the matching degree higher than 85%, and the composition of dissolved matters varies significantly during the immersing time. While using SPME as the sample pretreatment method, the identified chemicals are much more than those reported previously. Moreover, 22 chemicals with relative abundance higher than 1% and the detection rate higher than 80%, are investigated quantitatively. The result shows that 7 components increase by the increasing immersing times, and reach the highest concentration of 5 848 ng/mL after 30 days. Nevertheless, the COD and TN values of the dissolved matters are inferior to Ⅴ class of surface water quality standards of China, while NH+4-N is inferior to Ⅲ class after 15 days. Obviously, more attentions should be paid for the effects of dissolved matters of eucalyptus Leaves to the aquatic environments.

    Study on the metabolites by solid fermentation from the mangrove endophytic fungus Alternaria sp. R6
    CHEN Min;ZHOU Danli;ZHU Junjie;FENG Zihao;QIU Yuankun;SHE Zhigang;LI Chunyuan
    2016, 55(3):  117-121. 
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    To study the metabolites of the mangrove fungus Alternaria sp. R6, the compounds were isolated by silica gel and Sephadex LH-20 column chromatography. Their structures were characterized by spectroscopy. The antifungal activities were investigated using the broth tube dilution method. Ten compounds, including (4R,5R)-4,5-dihydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one(1), (4R, 5S,6R)-4,5,6-trihydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one (2), xylariacyclone B (3), (4S, 5S,6S)-5,6-epoxy-4-hydroxy-3-methoxy-5-methyl-cyclohex-2-en-1-one (4), LL-C10037α(5), 1- hydroxy-8-(hydroxymethyl)-6-methoxy-3-methyl-9H-xanthen-9-one (6), 8-hydroxy-6- methyl- 1- meth-oxycarbonyl-xanthen-9-one (7), 8-dimethoxy-1,6-dimethoxycarbonyl-xanthen-9-one(8), ergosterol and ergosterolperoxide, were isolated from the methanol extracts. Among them, the compounds 1, 2, 3, 4, 5, 6 and 8 were obtained from Alternaria sp. for the first time. The compounds 6 and 8 showed good inhibitory activity against Fusarium graminearum Schw. and Colletotrichum musae (Berk. & M. A. Curtis) Arx. While the compound 2 only showed good inhibitory activity against the latter.

    The chiral transition mechanism of α-Ala confined in the different sizes of armchair SWCNT——Based on using amino as the proton transfer bridge
    LI Xiaoping;YAN Hongyan;MEI Zeming;WANG Zuocheng
    2016, 55(3):  122-130. 
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    The reaction mechanism of α-Ala chiral transition confined in different sizes of armchair SWCNT, which achieved based on using amino as the proton transfer bridge, was studied using the combined method of quantum mechanics and molecular mechanics at ONIOM (MP2/6-311++G(3df, 3pd):UFF)//ONIOM(B3LYP/6-31+G(d, p):UFF). The result of reaction channel analysis showed that armchair SWCNT two channels a and b in different sizes were both found in the chiral transition reaction. Moreover, the amino N was used as a transfer bridge of the proton in the chiral carbon in the channel a, and that in the channel b the carbonyl O and amino N were successively used as a transfer bridge of the proton in the chiral carbon. The calculation of potential energy surface showed that the smaller the pore size of SWCNT, the lower the reaction energy barrier. In SWCNT(5,5), the highest energy barrier (198.7 kJ·mol-1 ) was obviously lower than that (266.1 kJ·mol-1) of the single in channel a, and the highest energy barrier (285.0 kJ·mol-1) in channel b was significantly lower than that (326.6 kJ·mol-1) of the single. The result implied that the chiral transformation of α-Ala in the biological channels was mainly achieved by using amino as the proton transfer bridge, and that for the chiral transition of α-Ala, the confinement effect of the smaller size of nanotubes reactor in catalysis was more obvious.

    Analysis of DOM decomposition in environmental water treated by potassium ferrate using three-dimension exaction and emission matrix fluorescence spectroscopy
    CHEN Jianghan;LEI Yongqian;ZHOU Qiaoli
    2016, 55(3):  131-134. 
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    The three-dimension exaction and emission matrix fluorescence spectroscopy (3D EEM) was used to investigate the decomposition of DOM in environmental water treated by potassium ferrate. The results showed the changed regular of fluorescence of several organic maters during the treatment process, and the pH value performed an important role on the fluorescent intensity and position. The intensity of fluorescence experienced a quick-decay process and a slowdecay process. Such 3D EEM method can be used as a simple and quick method for the online monitor of variety fluoresce maters.

    Preparation of N16, an anti-osteoporosis protein,by biological engineering method
    XU Zhenyan;AU Kayee; WONG Kalok;SHAW Pangchui;SU Weiwei ;PENG Wei ;WANG Yonggang;LI Peibo
    2016, 55(3):  135-138. 
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    N16 protein is a potential agent for treatment of osteoporosis due to its favorable anabolic and antiresorptive effects on bone. Our work described a biological engineering method to prepare N16. To build the high level expression system, pRHis plasmid was transformed into BL21 (DE3) plysS strain. The present study has established a complete N16 preparation process with simple protein expression and purification procedures. This procedure can improve N16 protein production, reduce the cost and shorten the cycle, which will facilitate further studies on the functions of N16.

    Application of compound microecological preparation in yellow-feathers black-bones chickens
    MEN Wenhui;HU Zhiming;LIN Lianbing;DENG Xianyu
    2016, 55(3):  139-144. 
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    This experiment was conducted on study the effects of compound microecological preparation (CMP) on growth performance, immune function, antioxidation level and intestinal microflora of yellow-feathers black-bones chickens. Three hundred healthy 1-day-old chickens were randomly assigned to 5 dietary treatments (probiotics 1, probiotics 2, probiotics 2 + Chinese herbal medicine + maggot powder, Chinese herbal medicine + maggot powder and control group). The results were shown as follows: ① CMP increased ADG and F/G significantly, but had no significant effects on ADFI. ② Compared with the control group, CMP stimulated the development of organs like thymus, spleen and bursa of fabricius significantly and increased the content of IgG by 46%, the activity of LZM by 69%. ③ CMP enhanced the levels of antioxidation by increasing the activity of GSH-PX, CAT and SOD, the effect is more pronounced in the liver. ④ CMP reduced the intestinal pH distinctly and improved the probiotics proportion of intestinal microflora by 20.2%.

    Formula principles of Kouyanqing granule for the anti-inflammatory effects
    ZHENG Yanfang;LI Chuyuan;LIU Hong;WANG Deqin;PENG Wei;WU Zhong;SU Weiwei
    2016, 55(3):  145-150. 
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    The present study was designed to reveal the formula principles of Kouyanqing granule (KYQG) for the antiinflammatory effects. According to uniform design, eleven KYQG samples with different herb proportions were prepared on the premise of the original formula proportions. Experiments with Human oral keratinocytes (HOK) were conducted to evaluate KYQG samples-anti-inflammation effects. Grey relational analysis approach was used to analyze the relevance between the five herbs and the biological effects in the KYQG samples. The results demonstrated that Flos lonicerae (flower bud of Lonicera macranthoides Hand.Mazz.) is the key herb performing an obviously higher contribution to the regulation of TNF-α, IL-8, IL-6, and IL-1β. Radix scrophulariae (root of Scrophularia ningpoensis Hemsl.), Radix ophiopogonis (root of Ophiopogon japonicus (Thunb.) Ker-Gawl.), and Radix asparagi (root of Asparagus cochinchinensis (Lour.) Merr.) are closely related to IL-6 and IL-1β. Radix et rhizoma glycyrrhizae (root of Glycyrrhiza uralensis Fisch.) contributes more to the suppression of TNF-α. The four herbs noted above play a role in strengthening the antiinflammatory effects of Flos lonicerae (flower bud of Lonicera macranthoides Hand.Mazz.).